Preliminary Test on a New Type of Antibacterial Substance  

一种新型抗菌活性物质的初步研究（英文）

作　　者：易凌霄 陈智勇[2] 董伟[3] 

机构地区：[1]湖南农业大学国际学院,湖南长沙410128 [2]湖南农业大学生物科学技术学院,湖南长沙410128 [3]湖南农业大学动物医学院,湖南长沙410128

出　　处：《Agricultural Science & Technology》2015年第11期2428-2432,共5页农业科学与技术（英文版）

基　　金：Supported by Guangdong Scientific and Technological Bureau(2012B020401012)~~

摘　　要：Application of high-speed centrifugation and high-performance liquid chro- matography to separation and purification obtained a new type of natural antibacteri- al substance from Escherichia co/i cell. Against Staphy/ococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus, Pseudomonas, solanacearum the minimal inhibitory concentration （MIC） was 2.5, 5, 0.625 iJg/ml, 0.625 and 1.25 g/ml, the minimum bactericidal concentration （MBC） was 5, 5, 1.25, 2.5, 2.5 g/ml, respectively. Composition identification of chemical method and mass spectrometry shows that the antibacterial active substance alkaloids; and the safety evaluation on the model plant Arabidopsis shows that the antibacterial active substance had no inhibitory and adverse effects on the growth and development of plants. The experimental results show that the antibacterial active substance is a broad-spectrum, high efficiency, safe antibacterial natural product, with the prospect of development and utilization of alternative antibiotics.应用高速离心和高效液相色谱法分离纯化技术,从大肠杆菌细胞裂解液中获得了一种新型的天然抗菌活性物质。体外抑菌试验结果显示:该活性物质对金色葡萄球菌、大肠杆菌、巴氏杆菌、链球菌、青枯病菌的最小抑菌浓度(MIC)分别为2.5、5、0.625、0.625、1.25μg/ml,最低杀菌浓度(MBC)分别为5、5、1.25、2.5、2.5μg/ml;化学法和质谱法进行成分鉴定的结果证明该抗菌活性物质为生物碱:对模式植物拟南芥的安全性评价结果显示该抗菌活性物质对植物的生长发育无抑制作用和不良影响。上述实验结果表明:该抗菌活性物质是一种广谱、高效、安全的新型抗菌天然产物,具有可替代抗生素类药物的开发利用前景。

关 键 词：Antibacterial substance ALKALOID Escherichia coli Blight ARABIDOPSISTHALIANA 

分 类 号：S482.2[农业科学—农药学]