Determination of β-sitosterol Content in Ethnodrug Guoshangye  

民族药果上叶中β-谷甾醇的含量测定（英文）

作　　者：马四补[1,2] 张应蓉 屈相玲[3] 靳凤云[2] 

机构地区：[1]中央民族大学,北京100081 [2]贵阳中医学院,贵州贵阳550002 [3]贵阳中医学院第二附属医院,贵州贵阳550001

出　　处：《Agricultural Science & Technology》2015年第11期2546-2548,共3页农业科学与技术（英文版）

基　　金：Supported by Traditional Chinese Medicine and Ethnic Minority Medicine Technique Research Subject of Guizhou Administrative Bureau of TCM(QZYY2012-34);贵州省中医药管理局中医药,民族医药科学技术研究课题(QZYY2012-34)

摘　　要：[Objective] The contents of β-sitosteml in Guoshangye from different habi- tats were determined. [Metlmd] UPLC-ELSD was adopted to determine the contents of β-sitosteml in Guoshangye from different habitats, in which BEH C18 column （2.1 mmx50 ram, 1,7 urn） was used with a mobile phase of methanol-water at a flow rate was of 0.3 ml/min, and the column temperature was of 30 ℃; and an evaporative light-scattering detector was used, the temperature in drift tube was of 90 ℃ and the pressure of N2 was of 20 psi. [Concludoa] This research provided theoretical foundation for the determination of β-sitosterol in Ethnodrug Guoshangye.[目的]测定不同产地果上叶中β-谷甾醇的含量。[方法]采用超高效液相色谱蒸发光散射检测器(UPLC-ELSD)测定不同产地果上叶中β-谷甾醇的含量。色谱条件为:BEH C_(18)柱,甲醇-水为流动相,蒸发光散射检测器检测参数为漂移管温度90℃,载气(N2)20psi,流速为0.3 ml/min,柱温30℃。[结果]不同产地的果上叶中β-谷甾醇的含量不同,3、4批次含量较高。该操作在常温下进行,灵敏度高,操作简便、快速。[结论]该研究为果上叶药材果上叶中β-谷甾醇的含量测定提供了理论依据。

关 键 词：Β-SITOSTEROL Ethnodrug Guoshanye DETERMINATION 

分 类 号：R284[医药卫生—中药学]