氧化苦参碱对坐骨神经结扎小鼠的镇痛作用及其对CaMKⅡ受体表达的影响  

Effect of oxymatrine on Ca MKII expression in sciatic nerve ligation-induced neuropathic pain in mice

作　　者：杜娟 张逢源[2] 许红霞 王娅娜 朱佳佳 顿铃露 余建强[3] 

机构地区：[1]宁夏医学科学研究所 [2]宁夏医科大学基础医学院 [3]宁夏医科大学药学院,宁夏银川750001

出　　处：《中国药理学通报》2015年第12期1719-1724,共6页Chinese Pharmacological Bulletin

基　　金：宁夏自然科学基金资助项目(No NZ13227)

摘　　要：目的研究氧化苦参碱(OMT)对神经病理性疼痛小鼠的镇痛作用及其机制。方法采用坐骨神经结扎模型(CCI)机械缩足反射法观察OMT的镇痛作用,并分析其镇痛作用与Ca MKII受体的关系。结果 1给小鼠腹腔注射160、80 mg·kg^(-1)OMT可明显延长小鼠的机械缩足反射期(P<0.05)。2给小鼠腹腔注射160、80、40 mg·kg^(-1)OMT可明显降低小鼠的冷缩足反射次数(P<0.05)。3 OMT能够跟CaMKⅡ拮抗剂KN-93和AIP发挥协同作用。4模型组小鼠的pCaMKⅡ蛋白表达较假手术组明显升高(P<0.01);与模型组比较,OMT(160 mg·kg~(^(-1)))组可明显降低p CaMKⅡ蛋白的表达(P<0.01)。结论 OMT对神经病理性疼痛具有镇痛作用,其镇痛作用可能与CaMKⅡ有关。Aim To observe the analgesic effect of oxymatrine( OMT) and its mechanism. Methods A peripheral mononeuropathy was produced in adult mice by placing loosely constrictive ligatures around the common sciatic nerve. The antinociceptive effects of the OMT were assessed in mechanical allodynia and cold allodynia tests. The CAMKII inhibitor KN-93 and AIP were adopted to investigate the influence of OMT on the analgesic effect and analyze its analgesic mechanisms. Western blot was used to evaluate the expressions of t CaMKⅡ and p CaMKⅡ protein. Results The intraperitoneal administration of OMT( 160,80 mg ·kg^(-1)) increased the paw withdrawal threshold in the mechanical allodynia test( P < 0. 05),OMT( 160,80,40 mg·kg^(-1),ip) remarkably decreased the paw lifts in the cold allodynia test( P < 0. 05). Ith KN-93( 1. 25 μg /site),AIP( 0. 02 μg /site) significantly enhanced the analgesic effect of OMT( 35 mg · kg^(-1))( P < 0. 01). Protein expression of p CaMKII was decreased by OMT( 160 mg·kg^(-1)). Conclusion OMT has significant protective effects on chronic constriction injury( CCI) in mice,and the effective mechanism of OMT inhibits the expression of CaMKⅡ receptor.

关 键 词：氧化苦参碱 神经病理性疼痛 坐骨神经结扎 镇痛作用 CaMKⅡ 小鼠 

分 类 号：R-332[医药卫生] R284.1