栀子柏皮汤及含栀子配伍组对免疫性肝损伤小鼠的保护作用  

Study on the protective effect in immunological liver injury in mice of Zhizi Baipi Soup and its disassembled prescription

作　　者：杨扬[1,2,3] 吴小琴[1,2,3] 李小枫[1,2,3] 李晚霞[1,2,3] 黄成[1,2,3] 李俊[1,2,3] 

机构地区：[1]安徽医科大学药学院 [2]安徽医科大学肝病研究所 [3]安徽省创新药物产业共性研究院,安徽合肥230032

出　　处：《中国药理学通报》2015年第12期1764-1769,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81473268;81273526);教育部博士点基金项目(No 20123420120001);安徽省自然科学基金项目(No 1308085MH145)

摘　　要：目的探讨栀子柏皮汤各及含栀子配伍组对刀豆蛋白(ConA)诱导免疫性损伤小鼠的保护作用及可能作用机制。方法观察栀子柏皮汤及各含栀子配伍组对尾静脉注射刀豆蛋白(ConA,20 mg·kg^(-1))诱发的免疫性肝损伤小鼠肝脏病理变化及血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活性、肝匀浆中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。酶联免疫吸附实验(ELISA)测定血清中干扰素γ(IFN-γ)、肿瘤坏死因子α(TNF-α)、白细胞介素-4(IL-4)和白细胞介素-6(IL-6)的水平。Western blot法检测与免疫炎症相关的NF-κB-p65的蛋白表达情况。结果栀子柏皮汤及各含栀子配伍组均能够降低血清ALT、AST及肝组织中MDA的含量;提高肝组织中SOD活性;降低NF-κB-p65及NF-κB-p-p65的表达,其中栀子柏皮汤全方组对免疫性肝损伤小鼠保护作用最佳。结论栀子柏皮汤对ConA诱导的免疫性肝损伤小鼠具有保护作用,其作用机制可能与调控免疫反应的NF-κB通路有关。Aim To investigate the effect of Zhizi Baipi soup and its disassembled prescription containing Zhizi on protecting concanavalin( ConA)-induced immunological liver injury in mice and explore the possible protective mechanism. Methods The model of mouse immunological liver injury induced by( ConA,20 mg·kg^(-1)) was used to observe the effects of Zhizi Baipi soup and its disassembled prescription containing Zhizi by gavage administration. Results Zhizi Baipi Soup and its disassembled prescription containing Zhizi were able to reduce the level of serum ALT,AST and MDA content,improve liver tissue SOD activity and reduce the expression of NF-κB-p65 and NF-κB-p-p65, in which all parties of Zhizi Baipi Soup Group had best effect on immunological liver injury in mice. Conclusion Zhizi Baipi Soup and its disassembled prescription containing Zhizi have remarkably protective effect on ConA-induced immunological liver injury in mice,and the potential mechanism may be related to the regulation of the immune response pathway.

关 键 词：栀子柏皮汤 含栀子配伍组 刀豆蛋白 免疫性肝损伤 NF-κB-p65 NF-κB-p-p65 

分 类 号：R-332[医药卫生] R282.71