蜈蚣肠道菌抑菌活性和酶活性检测  

Detecting the Antimicrobial and Enzyme Activity of Centipede's Intestinal Bacteria

作　　者：曹艳茹[1,2] 岳朝元 刘子超[1] 余磊[3] 

机构地区：[1]昆明学院生命科学与技术系,云南昆明650214 [2]云南省高校特色生物资源开发与利用重点实验室,云南昆明650214 [3]昆明学院农学院,云南昆明650214

出　　处：《昆明学院学报》2015年第6期60-62,79,共4页Journal of Kunming University

基　　金：云南省应用基础研究计划资助项目(S2012FZ0030);昆明学院云南省高校特色生物资源开发与利用重点实验室开放基金资助项目(GXK2015)

摘　　要：随着微生物各项培养及鉴定技术的快速发展,动物的肠道微生物研究也取得了很大进展.蜈蚣是一种有毒的节肢动物,其药用价值很高.它的肠道微生物有什么功效,相关报道极少.采用3种培养基分离培养了其肠道微生物,共获得10株菌,其中有9种细菌,1种放线菌.最后对10株菌进行4种酶活性检测和6种致病菌抑菌活性检测.酶活性检测结果显示,W10具有纤维素酶活,W12具有蛋白酶活,W1,W4,W6,W10,W15和W22具有脂肪酶活,没有检测到有淀粉酶活的菌株.抑菌检测结果显示,没有菌株抑制茄病镰刀菌,W10能够抑制细极链格孢菌,W12,W16和W22能够抑制炭疽病菌,抑制甘薯黑斑病菌的阳性菌株有W4,W6,W10,W12,W15和W22,W14能够抑制小新壳梭孢病菌,抑制稻梨孢菌的阳性菌株有W4,W6,W15和W16.对微生物酶活性的研究可了解蜈蚣肠道内微生物的消化功能;而对微生物抑菌活性的研究可对蜈蚣毒素的分泌提供一些参考,同时为新药物的研究提供菌种来源.With the development of microbial identification technology and insect cultivation,the research on the gut microbe of the insect is developing fast.Centipede is one of poisonous arthropods,but its medicinal value is very high.Few is reported about the func-tion of gut microbe of centipede.Three kinds of culture media were used to isolate its intestinal microorganisms,and 1 0 strains of bacte-ria were obtained,including 9 kinds of bacteria and 1 actinomycete.Then the activity of protease,lipase,cellulase and amylase was de-tected for the 1 0 strains by using the plate ring method.Antimicrobial activities against six fungi were tested using agar well diffusion method.The result of enzyme activity detection showed that W1 0 has cellulase activity;W1 2 has the protease activity;W4,W6,W1 0, W1 5,W22 have the lipase activity and the amylase activity was not detected.Antimicrobial test results showed that no strain inhibited Fusarium solani;W1 0 could inhibit Alternaria alternate;W1 2,W1 6,W22 could inhibit Colletotrichum capsici;W4,W6,W1 0, W1 2,W1 5,W22,W1 4 could inhibit Neofusicoccum parvum and W4,W6,W1 5,W1 6 could inhibit Pyricularia oryzae.Enzyme activ-ity screening can understand the function of centipede gut microbes in the digestive.The study of antimicrobial activity can provide some reference for comprehending toxin secretion of centipede.The strains showed high antimicrorbial activities can be further researched for exploiting new compounds of pharmaceutical.

关 键 词：蜈蚣 肠道微生物 酶活性 抑菌活性 

分 类 号：R285[医药卫生—中药学]