Runx3、Smad4、Cdk2、p21在胃癌中的表达及其临床意义  

Expressions and Clinical Significance of Runx3,Smad4,Cdk2 and p21 in Gastric Cancer

作　　者：刘雷[1] 陈国昌[1] 宋振云[2] 郜恒骏[2] 陈卫昌[3] 

机构地区：[1]江苏省宜兴市人民医院消化内科,214200 [2]生物芯片上海国家工程研究中心,214200 [3]苏州大学附属第一医院消化科

出　　处：《胃肠病学》2015年第10期597-601,共5页Chinese Journal of Gastroenterology

摘　　要：背景:近年来,组织芯片技术已越来越多地应用于恶性肿瘤相关研究。TGF-β信号通路在恶性肿瘤的发生、发展中起重要作用。目的:应用组织芯片技术研究TGF-β信号通路相关分子Runx3、Smad4、Cdk2、p21在胃癌中的表达及其与胃癌临床病理特征和预后的关系。方法:收集130例行胃癌根治术患者的癌组织(n=130)癌旁组织(n=248)标本石蜡块,制成组织芯片,以免疫组化方法检测Runx3、Smad4、Cdk2、p21表达。结果:胃癌组织Runx3、Smad4、Cdk2、p21异常表达率分别为67.7%、35.4%、63.8%和70.0%,分别显著高于癌旁组织的14.1%、12.5%、18.1%和37.1%(P<0.05)。Runx3异常表达与胃癌组织学分型和淋巴结转移有关(P<0.05),Smad4和p21异常表达仅与胃癌组织学分型有关(P<0.05),Cdk2异常表达与胃癌组织学分型、淋巴结转移和TNM分期有关(P<0.05)。除Cdk2仅与Runx3相关外,Runx3、Smad4、p21在胃癌中的异常表达两两相关。Kaplan-Meier生存分析显示,Runx3、Smad4、p21异常表达组5年生存率分别显著低于相应正常表达组(P<0.05);Cox比例风险模型显示Runx3和Smad4为胃癌患者的独立预后因素。结论:Runx3、Smad4、Cdk2、p21可能在胃癌的发生、发展中起一定作用。由于四者间存在相互影响,Runx3和Smad4能否作为判断胃癌预后的指标尚待进一步研究。Background：Tissue microarray has been increasingly used in research of malignancies. It has been revealed that TGF-β signaling pathway contributes to the tumorigenesis and progress of malignancies. Aims：To determine the expressions of Runx3,Smad4,Cdk2 and p21,the key molecules in TGF-β signaling pathway by tissue microarray,and investigate their correlations with clinicopathological features and prognosis of gastric cancer. Methods：A total of 378 paraffin embedded tissue blocks,including 130 gastric cancer tissue and 248 para-cancer tissue from 130 patients undergoing radical resection of gastric cancer were obtained. Tissue microarray and immunohistochemistry were used to determine the expressions of Runx3,Smad4,Cdk2 and p21. Results：The aberrant expression rates of Runx3,Smad4, Cdk21 and p21 in gastric cancer tissue were significantly higher than those in para-cancer tissue（67. 7% ,35. 4% , 63. 8% and 70. 0% vs. 14. 1% ,12. 5% ,18. 1% and 37. 1% ,P〈 0. 05,respectively）. Aberrant expression of Runx3 was closely correlated with histological grade and lymph node metastasis of gastric cancer（ P〈 0. 05）,while aberrant expressions of Smad4 and p21 were correlated with histological grade only（P〈 0. 05）;aberrant expression of Cdk2 was correlated with histological grade,lymph node metastasis and TNM staging（P〈 0. 05）. Pairwise correlations were seen among aberrant expressions of Runx3,Smad4 and p21 in gastric cancer,while Cdk2 was correlated with Runx3 only. Kaplan-Meier survival curve showed that 5-year survival rates in Runx3,Smad4 and p21 aberrant expression groups were significantly lower than those in normal expression groups（P〈0. 05）. Furthermore,Cox proportional hazard model indicated that Runx3 and Smad4 were independent prognostic factors for gastric cancer. Conclusions：Runx3,Smad4,Cdk2 and p21＆amp;nbsp;might play pivotal roles in tumorigenesis and progression of gastric cancer. As interactions occurred among these four proteins,whether Runx3 and Smad4 could be used f

关 键 词：组织芯片 胃肿瘤 预后 RUNX3 SMAD4 CDK2 P21 

分 类 号：R735.2[医药卫生—肿瘤]