苹果皮提取物及有效成分C3G对小鼠乳腺恶性肿瘤生长的影响  

Effect of apple peel extract and its active components C3G on mouse's malignant breast tumour growth

作　　者：郭强[1] 汪心怡[2] 朱其苹 范楚苓 朱佩[1] 任海风 李菲菲[1] 汪思应[1] 

机构地区：[1]安徽医科大学基础医学院病理生理学教研室,合肥230032 [2]安徽医科大学临床医学院,合肥230032

出　　处：《安徽医科大学学报》2015年第12期1711-1715,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81302319);安徽省自然科学基金(编号:1308085MH121);安徽省留学回国人员科研项目(编号:2009-2011)

摘　　要：目的探讨苹果皮提取物及其有效成分矢车菊-3-葡萄糖苷(C3G)对乳腺恶性肿瘤生长的影响。方法建立E0771小鼠乳腺癌移植瘤模型,观察饮用苹果皮汁对小鼠乳腺恶性肿瘤生长的影响。免疫组化法分析饮用苹果皮汁与饮用普通无菌水组小鼠恶性肿瘤组织中CD31表达差异。HPLC法分析苹果皮提取物中的化学成分。3D Matrigel胶血管形成实验探讨苹果皮成分C3G对小鼠乳腺恶性肿瘤血管形成的影响。MTT法和Transwell实验观察C3G对血管内皮细胞SVEC增殖和迁移能力的影响。结果饮用含有1%和2%浓度的苹果皮汁无菌水对乳腺恶性肿瘤生长起到抑制作用。免疫组化法染色显示饮用苹果皮汁肿瘤中CD31表达低于未饮用苹果皮汁组。HPLC法分析显示C3G是苹果皮提取物的有效成分之一。3D Matrige胶实验显示C3G抑制小鼠乳腺癌细胞E0771血管生成。C3G血管形成抑制小鼠内皮细胞SVEC的迁移能力。结论苹果皮提取物及其有效成分C3G抑制小鼠乳腺癌生长,该作用可能通过抑制血管生成而实现。Objective To investigate the effect of the side （ C3 G） on malignant breast tumour growth in vivo. apple peel extract and its active ingredients cyanidin-3-gluco-Methods C57BL/6 mouse breast cancer model was built to observe the effect of drinking apple peel juice on mouse breast tumour growth. IHC was used to analyze the differ- ential expression of CD31 in the apple peel extract treated group and the control group. HPLC was used to analyze the chemical composition of apple peels extract. The effect of apple peel component C3 G on blood vessels formation of mouse breast cancer cell line E0771 was investigated through 3D Matrigel. The effect of C3G on proliferation and migration of SVEC were determined via MTY and Transwell. Results 1% and 2% concentration of apple peel juice inhibited the tumour growth in mouse breast cancer model. The expression of CD31 in apple peel extract trea- ted group was lower than that of control group indicated by IHC staining. C3 G was one of the effective components of the apple peel extract through HPLC analysis. C3G inhibited the blood vessels formation of mouse breast cancer cell line E0771 indicated apple peel extract and its by 3D Matrigel. The migration of SVEC cells was inhibited by C3G. Conclusion The effective component C3G inhibit the tumour growth of mouse breast cancer, which is possibly achieved by inhibiting angiogenesis.

关 键 词：乳腺癌 苹果皮 矢车菊-3-葡萄糖苷 

分 类 号：R363.2[医药卫生—病理学]