microRNA-21对肺癌A549细胞增殖和Smad7表达的影响  

Influence of miR-21 control Smad7 expression and lung cancer A549 cell line proliferation

作　　者：李斌[1] 潘跃银[1] 杜瀛瀛[1] 

机构地区：[1]安徽医科大学第一附属医院干部肿瘤科,合肥230022

出　　处：《安徽医科大学学报》2015年第12期1766-1769,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技攻关项目(编号:1301042214);安徽省2012年度科研计划项目(编号:12070403072);安徽省高校省级自然科学基金重点项目(编号:KJ2012A157)

摘　　要：目的探讨微小RNA-21(miR-21)对肺癌A549细胞中Smad7表达的调控和对肺癌A549细胞增殖的影响。方法常规培养肺癌细胞系A549,经脂质体转染miR-21模拟物和抑制物及阴性对照片段48 h后,采用Western blot、qRTPCR法检测Smad7基因及蛋白的表达水平,MTT法检测A549细胞增殖情况。结果转染miR-21模拟物后Smad7的mRNA和蛋白表达量降低(P<0.05),而转染miR-21抑制物后Smad7的mRNA和蛋白表达量升高(P<0.05);MTT结果显示转染miR-21模拟物后细胞的增殖能力明显增强(P<0.05),而转染miR-21抑制物后细胞增殖能力明显减弱(P<0.05)。结论通过下调miR-21可提高Smad7的mRNA和蛋白表达,进而抑制A549细胞的增殖。Objective To explore miR-21 regulation of Smad7 in lung cancer A549 cell line as well as its impact on the proliferation of lung cancer A549＂ cell line. Methods miR-21 mimic and inhibitors in lung cancer A549 cell line were transfected by using Lipofectamine 2000 Reagent. After 48 h, Western blot and qRT-PCR were applied to assess the expression of protein and mRNA of Smad7. MTY assay was used to determine the proliferation influence of the transfected lung cancer A549 cell line. Results Western blot and qRT-PCR showed that A549 cell trans- fected miR-21 mimic exhibited down-regulated Smad7 protein and mRNA expression, and A549 cell transfected miR-21 inhibitor exhibited up-regulated Smad7 protein and mRNA expression. The A549 cell proliferation activity decreased significantly after transfected miR-21 inhibitors. Conclusion miR-21 inhibitors can increase Smad7 protein and mRNA expression, and suppress the proliferation activity of lung cancer A549 cell significantly.

关 键 词：肺癌 微小RNA-21 SMAD7 增殖 

分 类 号：R734.2[医药卫生—肿瘤]