丹参酮ⅡA磺酸钠注射液对脑出血大鼠灶周内源性神经干细胞的影响研究  

Impact of Tanshinone ⅡA Sulfonate Sodium Injection on Peripheral Endogenous Neural Stem Cells of Rats with Cerebral Hemorrhage

作　　者：张毅[1] 方永军[1] 周锋[1] 胡亚莉[2] 柯尊华[1] 周振国[1] 罗卫 畅涛[1] 

机构地区：[1]陕西中医药大学附属医院脑外科,陕西省咸阳市712000 [2]陕西中医药大学

出　　处：《实用心脑肺血管病杂志》2015年第10期45-49,共5页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

基　　金：陕西省教育厅科学研究计划项目(自然科学专项)(11JK0697);陕西省科技厅科学技术研究发展计划项目(自然基金)(2012JM4004)

摘　　要：目的观察丹参酮ⅡA磺酸钠注射液对脑出血大鼠灶周内源性神经干细胞的影响。方法将78只Sprague Dawley大鼠随机分为正常组6只,假手术组24只,对照组24只及治疗组24只。治疗组、对照组、假手术组大鼠采用立体定向仪自体血注入法制备脑出血模型(假手术组大鼠只做刺入动作,不向脑内注血),治疗组大鼠造模后1 d开始腹腔注射丹参酮ⅡA磺酸钠注射液,对照组及假手术组大鼠造模后1 d开始腹腔注射0.9%氯化钠溶液,正常组大鼠常规饲养,采用免疫组化染色观察4组大鼠造模后第3天、7天、14天、28天灶周5-溴-2'-脱氧尿苷酸(Brd U)、神经元特异烯醇化酶(NSE)阳性细胞数。结果造模后第3天、14天及28天治疗组大鼠灶周Brd U阳性细胞数多于假手术组和对照组(P<0.05);造模后第7天治疗组大鼠灶周Brd U阳性细胞数多于正常组、假手术组及对照组,假手术组和对照组大鼠灶周Brd U阳性细胞数多于正常组(P<0.05)。治疗组大鼠灶周Brd U阳性细胞数在造模后第3天开始增多,到第7天时达峰值,此后逐渐下降。造模后第3天治疗组大鼠灶周NSE阳性细胞数多于假手术组,造模后第14天及28天治疗组大鼠灶NSE阳性细胞数多于假手术组和对照组(P<0.05);造模后第7天治疗组大鼠灶周NSE阳性细胞数多于正常组、假手术组及对照组,假手术组和对照组大鼠灶周NSE阳性细胞数多于正常组(P<0.05)。假手术组、对照组和治疗组大鼠灶周NSE阳性细胞数在造模后呈进行性增多,并于造模后第28天达峰值。结论丹参酮ⅡA磺酸钠注射液可促进脑出血大鼠灶周内源性神经干细胞的增殖并诱导其向神经元分化。Objective To observe the impact of tanshinone ⅡA sulfonate sodium injection on peripheral endogenous neural stem cells of rats with cerebral hemorrhage.Methods A total of 78 Sprague Dawley rats were randomly divided into groups A（n = 6）,B（n = 24）,C（n = 24）and D（n = 24）,stereotactic instrument and autologous blood injection method were used to prepare for cerebral hemorrhage model（rats of C group received puncture without intracerebral injection of autologous blood）,rats of A group were conventionally feed,rats of B group and C group received intraperitoneal injection of0.9% sodium chloride injection after 1 day of preparation,while rats of D group received intraperitoneal injection of tanshinoneⅡA sulfonate sodium injection after 1 day of preparation.Immumohistochemical staining method was used to observe the peripheral Brd U-positive cell counts and peripheral NSE-positive cell counts after 3 days,7 days,14 days and 28 days of preparation.Results After 3 days,14 days and 28 days of preparation,peripheral Brd U-positive cell counts of D group were statistically significantly more than those of B group and C group（P〈0.05）; after 7 days of preparation,peripheral Brd U-positive cell counts of D group were more than those of A group,B group and C group,and those of B group and C group were statistically significantly more than those of A group（P〈0.05）.Peripheral Brd U-positive cell counts of D group obviously increased at the third of preparation,and increased to the peak at the seventh day,and then gradually decreased.After 3 days of preparation,peripheral NSE-positive cell counts of D group were statistically significantly more than those of C group（P〈0.05）; after 14 and 28 days of preparation,peripheral NSE-positive cell counts of D group were more than those of B group and C group（P〈0.05）; after 7 days of preparation,peripheral NSE-positive cell counts of D group were more than those of A group,B group and C group,and those of B group and C group were statistically

关 键 词：丹参酮 脑出血 大鼠 神经干细胞 

分 类 号：R743.34[医药卫生—神经病学与精神病学]