miR-10b对胃癌细胞侵袭迁移的影响  

The Effect of MiR-10b on Gastric Cancer Cell Invasion and Migration

作　　者：李红米[1] 骆萍 邱杨[1] 钟晓鸣[1] 

机构地区：[1]江西省肿瘤医院,330029 [2]江西省南昌市第三医院,330009

出　　处：《实用癌症杂志》2015年第12期1761-1766,共6页The Practical Journal of Cancer

摘　　要：目的分析miR-10b在胃癌组织中的表达情况;探讨miR-10b ASO对胃癌细胞侵袭和迁移的影响。方法运用RT-PCR检测120例胃癌组织及对应正常组织中miR-10b的表达;通过miR-10b ASO降低胃癌细胞中miR-10b的表达,采用transwell实验和划痕实验观察miR-10b ASO对胃癌细胞侵袭和迁移能力的影响,同时运用western blot的方法检测侵袭相关蛋白的表达变化。结果在120例胃癌病例中,48.33%(58/120)的胃癌组织miR-10b表达明显高于19.17%(23/120)对应正常癌旁组织(P<0.05)。与空白对照组和转染随机ASO组相比,miR-10b ASO可以显著降低miR-10b的表达(P<0.05);Transwell实验和划痕实验结果显示转染miR-10b ASO后,胃癌细胞的侵袭迁移能力下降明显,同时相关侵袭蛋白RHOC表达下降(P<0.05)。结论 miR-10b在胃癌组织中表达上调,降低miR-10b表达可有效抑制胃癌细胞的侵袭和迁移。miR-10b有可能成为胃癌侵袭转移调控的新靶点。Objective To investigate the expression of miR-10b in gastric cancer tissues and the effects of miR-10b an-tisense oligonucleotides（ASO）on the invasion and migration of gastric cancer cells in vitro.Methods The expression of miR-10b in 120 cases of gastric cancer tissues and their adjacent tissues were detected by RT-PCR method.After transfection with miR-10b ASO,the biological effects of miR-10b in gastric cancer cells was measured by transwell assay and wound healing assay,and inva-sion-related protein expression was analyzed by western blot.Results The expression level of miR-10b in 48.33%（58/120） of the gastric cancer tissues was significantly higher than 19.17%（23/120）of normal tissues（P〈0.05）.Transwell and wound healing assay showed that invasion and migration ability decreased greatly after transfection with miR-10b ASO, furthermore, down-regulation of miR-10b resulted in obvious inactivation of RHOC.（P〈0.05）.Conclusion miR-10b is overexpressed in gastric cancer tissues.Reduce the expression of miR-10b can effectively inhibit the invasion and migration of gastric cancer cells. miR-10b may become a new target for the regulation of invasion and migration in gastric cancer.

关 键 词：MIR-10B 胃癌 反义单核苷酸 

分 类 号：R735.2[医药卫生—肿瘤]