稳定敲低MYH10基因细胞株的建立  

Establishment of Stable COS-7 Cell Strain with MYH10-knock-down

作　　者：周晨辰[1] 陆琤[1] 张鹏飞[1] 张硌[1] 

机构地区：[1]中国人民解放军307医院医学工程科,北京100071

出　　处：《医学研究杂志》2015年第11期51-53,共3页Journal of Medical Research

基　　金：国家自然科学基金青年科学基金资助项目(31400739);北京市自然科学基金资助项目(5144033)

摘　　要：目的构建稳定敲低MYH10基因的COS-7细胞株。方法在293TX细胞中进行病毒的包装,用获得的高效价慢病毒感染COS-7细胞,通过免疫印迹和荧光显微镜,检测病毒感染后COS-7细胞MYH10蛋白表达的变化。结果在构建的两种重组质粒中,MYH10-lentiviral shRNA-2在转染后表现出明显的干扰作用,感染后能明显下调COS-7细胞的MYH10蛋白表达。结论病毒感染的COS-7细胞可明显抑制COS-7细胞内源MYH10的表达,说明了稳定敲低MYH10基因的COS-7细胞株的建立,为进一步研究MYH10在相关疾病中的功能奠定了基础。Objective To establish COS -7 cell strain stable knockdown of MYH10 gene. Methods The lentiviral plasmids were transfected into 293TX cells to package into lentiviral particles. Then COS - 7 cells was transduced with the lentiviral particles. Western blot and fluorescence microscope were carried out to analyze the suppression of MYH10 in COS - 7 Cells. Results In the construction of two recombinant plasmid, MYH10 - lentiviral shRNA -2 showed significant interference after transfection, and can obviously reduced ex- pression of MYH10 in COS - 7 cells. Conclusion Infected COS - 7 ceils can inhibit the expression of the MYH10 COS - 7 ceils, indica- ting that a stable COS -7 cell strain has been successively established.

关 键 词：MYH10基因 COS-7细胞株 慢病毒感染 

分 类 号：R3[医药卫生—基础医学]