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机构地区:[1]辽宁医学院附属第一医院血液科,辽宁省锦州市121000
出 处:《实用医学杂志》2015年第22期3678-3681,共4页The Journal of Practical Medicine
基 金:辽宁省自然科学基金(编号:2013022031)
摘 要:目的:证明肿瘤坏死因子-α(TNF-α)可以促进转化生长因子-β(TGF-β)降低原代白血病细胞Gli2的表达。方法 :(1)Western blot检测白血病细胞Gli2蛋白的表达。(2)不同浓度TGF-β1和TNF-α分别作用于原代白血病细胞后,检测Gli2基因或蛋白以及TGF-βRⅠ和TGF-βRⅡ蛋白的表达。(3)TGF-β1、TNF-α及SIS3作用于原代白血病细胞24 h后,检测Gli2蛋白的表达。结果:(1)3株原代白血病细胞均有Gli2蛋白的表达。(2)1~10 ng/m L TGF-β1作用于原代白血病细胞12~24 h,与对照组相比Gli2基因m RNA表达降低明显;5 ng/m L TNF-α则没有显著变化。(3)5 ng/m L TNF-α作用于原代白血病细胞24 h后,与对照组相比TGF-βRⅠ和TGF-βRⅡ蛋白表达均有增高。(4)单独应用TGF-β以及联合应用TNF-α和TGF-β作用24 h后,与对照组相比Gli2基因的表达均降低明显,而且TNF-α+TGF-β组Gli2基因的表达较TGF-β组降低更明显。结论:联合应用TNF-α和TGF-β比单独应用TGF-β能进一步减少原代白血病细胞Gli2基因的表达。Objective To investigate whether TNF-α can promote, but TGF-β can reduce Gli2 expression in primary leukemia cells. Methods (1)Western blot was used to detect Gli2 protein expression in leukemic cells. (2)Primary leukemia cells were treated with TGF-β1 or TNF-α, and expressions of Gli2 and TGF-βR were detected. (3)Primary leukemia cells were treated with TGF-β1, TNF-α or/and SIS3, and Gli2 protein expression was detected. Results (1)The primary leukemia cells could express Gli2 protein. (2)Significant reduction of Gli2 mRNA was observed in 1 - 10 ng/mL TGF-β1-treated primary leukemia cells for 12 - 24 h, No significant differences of Gli2 mRNA was found between the control group and TNF-α group. (3)Protein expresson of TGF- βR I and TGF-βR 11 in 5 ng/mL TNF-α-treated primary leukemia cells for 24 h was higher than that of the control group. (4)Gli2 portein expression was lower in TGF-β1 with or without TNF-α-treated primary leukemia cells for 24 h than that in the control group, and Gli2 portein expression in the TGF-β plus TNF-α group was lower than that in TGF-β group. Conclusion Combinations of TGF-β and TNF-α can efficiently inhibit Gli2 expression than TGF-β alone in primary leukemia cells.
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