棘托竹荪菌托抑菌物质提取、分离及活性组分  

Extraction,Separation and Composition Analysis of the Antibacterial Components from Volva of Dictyophora echinovolvata

作　　者：林陈强[1] 林戎斌[1] 陈济琛[1] 张慧[1] 林新坚[1] 

机构地区：[1]福建省农业科学院土壤肥料研究所农业资源与环境研究中心,福州350013

出　　处：《天然产物研究与开发》2015年第11期1910-1916,共7页Natural Product Research and Development

基　　金：福建省科技厅重点项目(2013Y1003);福建省公益类科研院所项目(2014R1022-1)

摘　　要：以棘托竹荪(Dictyophora echinovolvata)菌托为研究对象,提纯菌托提取物有效抑菌天然产物,分析其抑菌活性组成。选择最佳提取溶剂及指示菌,采用色谱层析法分离纯化,GC-MS检测分析活性组分。结果表明:乙酸乙酯提取物对金黄色葡萄球菌、枯草芽孢杆菌、大肠杆菌、蜡状芽孢杆菌、标准摩根氏菌、肠炎沙门氏菌均有较好的抑制效果;以金色葡萄球菌为指示菌,乙酸乙酯提取物的抑菌作用对温度、紫外线均有较好的稳定性;对提取物进行分离,获得2个有抑菌活性组分DEVⅠ、DEVⅡ,采用GC-MS分析DEVⅠ、DEVⅡ中的化学组分,DEVⅠ中相对含量1%以上的成分有20个;DEVⅡ中相对含量在1%以上成分6个,其中具有抑菌活性的成分为2-呋喃甲酸和肉桂酸,相对含量分别为25.6%和54.2%,推测可能为棘托竹荪菌托抑菌物质的主要组成成分。Components from volva of Dictyophora echinovolvata were extracted by different extraction solvent and their an- timicrobial effects were determined using Staphylococcus aureus , Bacillus subilis , Escherichia coli , Bacillus cereus, Mor- ganella fulton, Salmonella enteritidis as indicator bacteria. In addition, the components with highest antibacterial activity were separated by column chromatography and the purified fractions were determined by gas chromatography-mass spec- trometry （GC-MS）. Results showed that the ethyl acetate extract from volva had highest antibacterial effect on the above pathogenic bacteria and was more stable for temperature and ultraviolet radiation. The ethyl acetate extract was then fur- ther purified to afford 2 antibacterial components, namely DEV I and DEVII. Through GC-MS analysis, twenty-six com- ponents with the relative content of more than 1% were identified in DEV I and DEVII, respectively. The major antibac- terial components of DEVII were 2-furoic acid with relative contents of 25.6% and cinnamic acid with relative contents of 54.2%. These two components were possibly the major antibacterial components from volva of D. echinovolvata.

关 键 词：棘托竹荪 菌托 抑菌活性物质 GC-MS 

分 类 号：S646.8[农业科学—蔬菜学]