Ghrelin对小鼠认知功能的影响及机制探讨  

Effect and Mechanism of Ghrelin on Cognitive Function in Mice

作　　者：陈任[1] 方姝晨 宁宇[2] 范里[1] 陈家禄[1] 

机构地区：[1]武汉大学人民医院脊柱外科,武汉430000 [2]苏州市吴江区中医医院骨科,苏州215200

出　　处：《神经损伤与功能重建》2015年第6期471-473,共3页Neural Injury and Functional Reconstruction

摘　　要：目的:探讨Ghrelin对小鼠认知功能的影响及机制。方法:野生型(WT)组和CGRP基因敲除(CGRP-/-)组小鼠各10只,且各分为对照亚组和实验亚组各5只,均腹腔注射100μL含0.1μg/L Gherlin的PBS试液(实验亚组)或空白PBS试液(对照亚组),进行Morris水迷宫实验及记忆探索实验,并测定其海马趾组织中CGRP、IGF-1及IGF-1 m RNA含量。结果:WT组中,实验亚组与对照亚组比较,逃避潜伏期时间缩短,目标区域停留时间百分比增加,CGRP、IGF-1及IGF-1 m RNA的含量较高(P<0.05);CGRP-/-组中,实验亚组与对照亚组相比差异无统计学意义(P>0.05);与CGRP-/-组实验亚组比较,WT组实验亚组注射第4、5天的逃避潜伏期较低,目标区域停留时间百分比较高,CGRP、IGF-1及IGF-1 m RNA含量较高,差异有统计学意义(P<0.05)。结论:Gherlin可能通过促进海马体释放CGRP,使得海马的IGF-1产量增加,由此提高小鼠的认知能力。Objective：To explore the effect and mechanism of Ghrelin on the cognitive function in mice. Methods：Ten wild-type mice （WT group） and 10 CGRP-gene knockout mice （CGRP-/- group）were equally divided as the control subgroup （n=5） and the examination subgroup （n=5）. Mice were treated by intraperitoneal injection of 100μL of 0.1 μg/L Ghrelin solution dissolved in PBS （examination subgroup） or with PBS alone （control subgroup）. The Morris maze task and probe test were examined in both groups, and then the tissue levels of CGRP, IGF-1 and IGF-1 mRNA in the hippocampus were determined. Results： In the WT group, the escape latency of the examina-tion subgroup was shortened, the time spent in the target area of the examination subgroup was significantly en-hanced, the tissue levels of CGRP, IGF-1 and IGF-1 mRNA were significantly up-regulated （P〈0.05） compared to those in the control subgroup. There was no statistically significance between the two subgroups in CGRP-/- group （P〉0.05）. Compared with the examination subgroup of CGRP-/- group, the escape latency of the examination sub-group of WT group was shorter on days 4 and 5, and the time spent in the target area was longer, and the tissue lev-els of CGRP, IGF-1 and IGF-1 mRNA were significantly higher （P〈0.05）. Conclusion： Ghrelin may increase the hippocampal expression of IGF-1 via increase of CGRP release from the hippocampus, thus cognitive function was improved.

关 键 词：GHRELIN 胰岛素样生长因子-1 认知功能 

分 类 号：R741[医药卫生—神经病学与精神病学] R741.02[医药卫生—临床医学]