骨髓间充质干细胞SCA-~1+/CD45^+/CD31^+亚群的归巢能力  

Homing ability of the SCA-1^+/CD45^+/CD31^+ subgroup of bone marrow mesenchymal stem cells

作　　者：贺继刚[1] 李洪荣[1] 桂龙升[1] 李永武[1] 严丹[1] 王平[1] 

机构地区：[1]云南省第一人民医院心脏大血管外科,云南省昆明市650032

出　　处：《中国组织工程研究》2015年第41期6572-6578,共7页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81460073);云南省科技厅-昆明医科大学应用基础研究联合专项(2014FB089)~~

摘　　要：背景:自2003年,美国FDA率先批准自体骨髓干细胞移植治疗心肌梗死性疾病以来,已有大量临床及基础研究报道,但报道结果却不尽相同,其中很重要的一点就是干细胞无法有效到达心肌损伤部位(即干细胞归巢)。目的:探讨小鼠骨髓间充质干细胞各亚群在心肌修复中的归巢能力。方法:以小鼠心肌干细胞表面分化抗原筛查小鼠骨髓间充质干细胞,再以CD45、CD31为标准分选得到小鼠骨髓间充质干细胞4个亚群。采用Transwell小室检测各亚群组细胞的归巢能力。将各亚群细胞注入心肌梗死48 h小鼠体内,注射后48,96 h及7 d处死小鼠取其心脏完成小动物活体成像并检测其荧光强度。结果与结论:小鼠骨髓间充质干细胞SCA-1+/CD45+/CD31+亚群归巢能力优于其他亚群。小动物活体成像提示干细胞注射48 h,96 h及7 d时SCA-1+/CD45+/CD31+亚群平均荧光强度优于其他亚群,SCA-1+/CD45+/CD31+亚群迁移率最高,说明SCA-1+/CD45-/CD31-群体有向受损心肌归巢的趋势。BACKGROUND： Since the FDA was the first to approve autologous bone marrow stem cell transplantation for treatment of myocardial infarction in 2003, there has a large number of clinical and basic research reports. However, their conclusions are different and stem cell homing is a key point. OBJECTIVE： To explore the homing abilities of different subgroups of mouse bone marrow mesenchymal stem cells in myocardial regeneration. METHODS： After mouse bone marrow mesenchymal stem cells were detected using a mouse cardiac stem cell surface differentiation antigen, four cell subgroups were separated on the basis of CD45 and CD31. The homing abilities of the four subgroups were assayed in a Transwell chamber in vitro. The different cell subgroups were injected into the model mice suffering from myocardial infarction for 48 hours. The mice were sacrificed at 48 hours, 96 hours, and 7 days after injection; the hearts were taken and analyzed through whole-body imaging and fluorescence intensity detection. RESULTS AND CONCLUSION： The SCA-1＋/CD45＋/CD31＋ subgroup exhibited the strongest homing ability. The whole-body imaging indicated that the fluorescence intensity of SCA-1＋/CD45＋/CD31＋ subgroup was higher than that of the other subgroups at 48 hours, 96 hours and 7 days after stem cell injection. The migration rate of SCA-1＋/CD45＋/CD31＋ subgroup was also the highest. These findings indicate that the homing ability of the SCA-1＋/CD45＋/CD31＋ subgroup of mouse bone marrow mesenchymal stem cells exhibit a homing trend to the damaged myocardial tissue.

关 键 词：心肌梗塞 骨髓 间质干细胞 细胞运动 组织工程 干细胞 骨髓干细胞 归巢 骨髓间充质干细胞 亚群 SCA-1 CD45 CD31 小鼠 心肌梗死 荧光强度 国家自然科学基金 

分 类 号：R394.2[医药卫生—医学遗传学]