CD133^+卵巢癌干细胞样细胞在血管内皮细胞中的分化  

CD133^+ ovarian cancer stem-like cells differentiate into vascular endothelial cells

作　　者：蒋立艳[1] 楼湘莹[2] 王自能[2] 路妍妍[2] 高瑞萍[1] 

机构地区：[1]广东药学院临床医学院,广东省广州市510224 [2]暨南大学附属第一临床医学院妇产科,广东省广州市510632

出　　处：《中国组织工程研究》2015年第41期6623-6627,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:大量研究证实,新生血管形成在肿瘤的生长、浸润以及转移过程中发挥重要作用。目的:探讨CD133+卵巢癌干细胞样细胞向血管内皮细胞分化的特点。方法:通过无血清培养方法从卵巢癌A2780细胞株中成功诱导出CD133+卵巢癌干细胞样细胞,在体外接种于铺或不铺Matrigel基质胶的96孔板内,观察不同时间点CD133+卵巢癌干细胞样细胞和人脐静脉内皮细胞形成管腔样结构能力。通过裸鼠皮下移植实验,免疫荧光法观察CD133+卵巢癌干细胞样细胞在卵巢癌血管新生中的作用。结果与结论:CD133+卵巢癌干细胞样细胞和人脐静脉内皮细胞(阳性对照)在未铺Matrigel基质胶上并不能形成相应的管腔结构,且不表达内皮细胞标志物CD31,在Matrigel基质胶上能够形成相对稳定的管腔结构,CD31表达明显。CD133+卵巢癌干细胞样细胞接种裸鼠皮下成瘤后,可观察到肿瘤组织中有人源性CD31的表达。结果表明CD133+卵巢癌干细胞样细胞能够分化为血管内皮细胞,参与肿瘤血管重建。BACKGROUND： Numerous studies have confirmed that neovascularization plays an important role in the growth, invasion and metastasis of tumors. OBJECTIVE： To investigate the features of CD133＋ ovarian cancer stem-like cells differentiating into vascular endothelial cells. METHODS： CD133＋ ovarian cancer stem-like cells were successfully harvested from A2780 ovarian cancer cell lines using serum-free culture method, and incubated in vitro onto 96-well plates with or without Matrigel. Then, we observed the capacity of CD133＋ ovarian cancer stem-like cells and human umbilical vein endothelial cells to form tube-like structures at different time points. Through xenograft experiments, the role of CD133＋ ovarian cancer stem-like cells in the angiogenesis of ovarian cancer was observed using immunofluorescence staining. RESULTS AND CONCLUSION： CD133＋ ovarian cancer stem-like cells and human umbilical vein endothelial cells cultured with no Matrigel had no corresponding lumen formation, and could not express CD31. But those cultured with Matrigel had lumen formation and expressed CD31 significantly. After tumor formation, human-derived CD31 expression was observed in the tumors. These findings indicate that CD133＋ ovarian cancer stem-like cells can differentiate into vascular endothelial cells, and be involved in tumor revascularization.

关 键 词：干细胞 卵巢肿瘤 内皮细胞 细胞分化 组织工程 肿瘤干细胞 卵巢癌干细胞样细胞 CD133 血管内皮细胞 

分 类 号：R394.2[医药卫生—医学遗传学]