人脐带间充质干细胞对异位子宫内膜细胞增殖及凋亡的影响  

Effect of human umbilical cord mesenchymal stem cells on proliferation and apoptosis of ectopic endometrial cells

作　　者：王春梅[1] 李洁[1] 孙文萍[1] 吴德慧[1] 

机构地区：[1]青海红十字医院,青海省西宁市810001

出　　处：《中国组织工程研究》2015年第41期6628-6632,共5页Chinese Journal of Tissue Engineering Research

摘　　要：背景:间充质干细胞可以分泌多种细胞因子和生长因子,促进周围细胞的存活,发挥旁分泌作用。目的:探讨人脐带间充质干细胞对异位子宫内膜细胞增殖及凋亡的影响。方法:分离培养人脐带间充质干细胞和异位子宫内膜细胞,以异位子宫内膜细胞单独培养作为对照组,人脐带间充质干细胞与异位子宫内膜细胞共培养作为观察组。培养24,48,72 h时采用MTT法检测异位子宫内膜细胞增殖情况,流式细胞仪检测异位子宫内膜细胞凋亡情况,RT-PCR检测异位子宫内膜细胞PTEN基因表达情况。结果与结论:与对照组比较,培养24,48,72 h时观察组异位子宫内膜细胞增殖受到显著抑制,亚二倍体峰占细胞总数的比例显著升高(P<0.05)。随着时间的推移,观察组细胞抑制率逐渐下降,各时间点比较差异有显著性意义(P均<0.05)。与同期对照组比较,观察组细胞PTEN基因表达显著上调(P<0.05)。培养48,72 h观察组细胞PTEN基因表达显著高于培养24 h(P<0.05)。以上结果表明人脐带间充质干细胞可以通过上调PTEN基因表达,抑制异位子宫内膜细胞增殖,并促进其凋亡。BACKGROUND： Mesenchymal stem cells can secrete a variety of cytokines and growth factors that promote the survival of surrounding cells and play a paracrine role. OBJECTIVE： To investigate the effect of human umbilical cord mesenchymal stem cells on the proliferation and apoptosis of ectopic endometrial cells. METHODS： After isolation and culture, human umbilical cord mesenchymal stem cells and ectopic endometrial cells were co-cultured as observation group, and ectopic endometrial cells cultured alone served as control group. At 24, 48, 72 hours of culture, the proliferation and apoptosis of ectopic endometrial cells were detected by MTT and flow cytometry, respectively; RT-PCR was used to measure the expression of PTEN gene in ectopic endometrial cells. RESULTS AND CONCLUSION： At 24, 48 and 72 hours, the proliferation of ectopic endometrial cells in the observation was inhibited significantly as compared with the control group, and the hypodiploid peak ratio also increased significantly（P〈0.05）. Over time, the cell inhibition rate was gradually declined, and there were significant differences at different time points（all P〈0.05）. Compared with the control group, the expression of PTEN gene was up-regulated significantly in the observation group（P〈0.05）. In the observation group, the expression of PTEN gene at 48 and 72 hours was significantly higher than that at 24 hours（P〈0.05）. These findings indicate that in the human umbilical cord mesenchymal stem cells can inhibit the proliferation of ectopic endometrial cells in vitro and promote their apoptosis by up-regulation of PTEN m RNA expression.

关 键 词：脐带 间质干细胞 子宫内膜异位症 共同培养技术 PTEN磷酸水解酶 细胞增殖 细胞凋亡 组织工程 干细胞 脐带脐血干细胞 脐带间充质干细胞 PTEN基因 

分 类 号：R394.2[医药卫生—医学遗传学]