Ctip2在福尔马林引起的大鼠急性颌面部炎性疼痛中的表达变化  

Expression of Ctip2 in formalin-induced acute maxillofacial inflammatory pain in rats

作　　者：岳黎[1] 姜珊[1] 熊伟[2] 梅雪[1] 金幼虹[1] 

机构地区：[1]南昌大学附属口腔医院牙周科,江西南昌330006 [2]南昌大学附属口腔医院口腔预防科,江西南昌330006

出　　处：《牙体牙髓牙周病学杂志》2015年第11期659-664,共6页Chinese Journal of Conservative Dentistry

基　　金：江西省教育厅科技计划课题(12003268)

摘　　要：目的:探讨Ctip2在口颌面部炎性疼痛中的作用。方法:取成年健康雌性SD大鼠132只,随机分为3组;空白对照组大鼠(n=12)不作任何处理,生理盐水(n=60)和福尔马林组(n=60)各大鼠分别在其左侧上唇注射50μL生理盐水(n=60)或等量25 m L/L的福尔马林。注射结束后立即观察各组大鼠在45 min内的疼痛行为,并分别于注射后30 min及1、2、3、4 h各时间点取各组大鼠的脑干延髓段组织(n=12);然后分别采用免疫组化染色(n=4)、免疫荧光染色(n=4)以及RT-PCR(n=4)检测各大鼠Vc核中Ctip2的表达情况。结果:1注射25 m L/L福尔马林后的各大鼠均表现出典型的双时相性疼痛反应,生理盐水对照组仅在注射初期出现自发性痛行为反应,空白对照组无类似表现;2注射福尔马林后30 min、1 h和2 h各时间点大鼠Vc核中的Ctip2阳性颗粒数量均明显增多,且分布集中、染色较深(P<0.05);注射福尔马林后30 min、1 h各时间点的荧光阳性颗粒均明显增多(P<0.05);注射福尔马林后30 min时,其Vc核中Ctip2 mRNA表达水平即明显升高,2 h时达到峰值(P<0.05)。结论:Ctip2参与中枢神经系统对炎性疼痛刺激的调节。AIM： To investigate the changes of Ctip2 expression in caudal subnucleus of the spinal trigeminal nucleus（ Vc） in rats with formalin- induced orofacial pain. METHODS： 132 adult female SD rats were randomly divided into 3 groups. The rats in control group（ n = 12） received no injection,in formalin or saline group（ n = 60）were injected with 50 μL of 25 m L / L formalin or the same volume of 0. 9% saline into the left upper lips,respectively.The behavior of the rats was observed for 45 minutes. Their Vc were taken at 30,60,120,180 and 240 minutes after injection respectively,Citp 2 expression in Vc was examined by immunochemistry,immunofluorescence and RT-PCR.RESULTS： The rats scratched the injected sites after administration of formalin,but did not in saline or control groups. Ctip2 protein expression was significantly upregulated in the rats 30 min,1 h and 2 h after formalin injection.Ctip2 mRNA was remarkably increased and reached the peak 2 h after administration of formalin. CONCLUSION：Ctip2 participates in regulating inflammatory pain in central nerve system.

关 键 词：福尔马林 炎性疼痛 转录因子Ctip2 三叉神经脊束核尾侧亚核 

分 类 号：R780.2[医药卫生—口腔医学]