姜黄素对成骨细胞凋亡的影响  

Effect of Curcumin on Tumor Necrosis Factor α-induced Osteoblast Apoptosis

作　　者：王雪梅[1] 尚德浩[2] 

机构地区：[1]中国医科大学口腔医学院牙体牙髓科,中心实验室,辽宁省口腔医学研究所牙体牙髓病学研究室,沈阳110002 [2]中国医科大学口腔医学院种植中心,沈阳110002

出　　处：《中国医科大学学报》2015年第12期1094-1097,共4页Journal of China Medical University

基　　金：辽宁省科技计划项目(2013225090)

摘　　要：目的研究姜黄素对肿瘤坏死因子α(TNF-α)处理成骨细胞引起凋亡的影响。方法将原代培养的大鼠成骨细胞分为3组:对照组(不做处理)、TNF-α处理组(10 ng/m L TNF-α处理24 h)、姜黄素预处理组(10μmol/L姜黄素预处理2 h,10 ng/m L TNF-α处理24 h),通过Western blot方法检测Fas、TRAIL、caspase-2、caspase-3、caspase-8及Bcl-2的蛋白水平,荧光显微镜下观察并计数成骨细胞的凋亡情况。结果与对照组相比,12和24 h时TNF-α处理组caspase-2、caspase-3、caspase-8、Fas、TRAIL的蛋白水平均升高(P<0.01),Bcl-2的蛋白水平无明显变化(P>0.05);12和24 h时姜黄素预处理组caspase-2、caspase-3、caspase-8、Fas、TRAIL的蛋白水平均较TNF-α处理组明显降低(P<0.01)。20倍荧光显微镜下观察可见,对照组凋亡的成骨细胞数目非常少,TNF-α处理组凋亡的成骨细胞数目明显增多,姜黄素预处理组凋亡的成骨细胞数目明显减少。结论姜黄素可以抑制TNF-α引起的成骨细胞凋亡。Objective To study the effect of curcumin on tumor necrosis factor α （TNF-α） -induced osteoblast apoptosis. Methods Primary osteo- blast cells were divided into three groups ： control group （ no treated）, group treated with TNF-α （treated with TNF-α for 24 h ）, and group pretreated with curcumin （treated with curcumin for 2 h, and then treated with TNF-α for 24 h）. Western blot analysis was carried out with primary antibodies against caspase-2, caspase-3, caspase-8, Bcl-2, Fas, and TRAIL. Apoptosis cells were observed by immunofluorescence microscope. Results In group treated with TNF-α, the protein levels of caspase-2, caspase-3, caspase-8, Fas, and TRAIL increased （P 〈 0.01 ）, whereas the protein level of Bcl-2 remained unchanged （P 〉 0.05 ）. In group pretreated with curcumin, the protein levels of caspase-2, caspase-3, caspase-8, Fas, and TRAIL were lower than those in group treated with TNF-α （P 〈 0.01 ）. The number of apoptotic cells was dramatically increased after the administration of TNF-cc While the number of apoptotic cells decreased in the presence of curcumin. Conclusion The present study clearly demonstrated that curcumin can suppress osteoblast apoptosis induced by TNF-α.

关 键 词：肿瘤坏死因子Α 成骨细胞 凋亡 

分 类 号：R781.4[医药卫生—口腔医学]