聚谷氨酸基超分子水凝胶的制备和表征  

Fabrication and Characterization of PLGA-based Supermolecular Hydrogel

作　　者：李贵飞[1] 吴杰[1] 王波[1] 张伟骏[1] 颜世峰[1] 尹静波[1] 

机构地区：[1]上海大学材料科学与工程学院高分子材料系,上海200444

出　　处：《高等学校化学学报》2015年第12期2582-2589,共8页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:51373094;51173101;51473090);上海市科学技术委员会基础研究项目(批准号:15JC1490400);上海市高校青年教师培养计划资助~~

摘　　要：以接枝胆固醇的聚谷氨酸-聚乙二醇-聚谷氨酸三嵌段聚合物和接枝β-环糊精的聚谷氨酸为前躯体,通过主客体作用制备聚谷氨酸基超分子水凝胶.通过调控前躯体的浓度、胆固醇和环糊精的摩尔比,得到不同力学性能的水凝胶;改变三嵌段聚合物中聚谷氨酸的分子量,调控聚谷氨酸基超分子水凝胶的力学强度、微观形貌、溶胀行为和降解性能.研究结果表明,当聚合物浓度为150 g/L、环糊精和胆固醇的摩尔比为1∶1时,水凝胶的储存模量最大;聚谷氨酸的分子量越大,水凝胶的储存模量越大;水凝胶平均孔径10-30μm,溶胀度16%-30%,降解时间为40-72 d.水凝胶细胞毒性实验结果表明超分子水凝胶具有良好的生物相容性.Supermolecular hydrogels composed of cholesterol-modified triblock polymers poly （L-glutamic acid） -poly（ ethylene glycol ） -poly （ L-glutamic acid ） [ （ PLGA-b-PEG-b-PLGA ） -g-Chol ] and β-cyclodextrins （β-CD）-modified poly（ L-glutamic acid）（PLGA-g-β-CD） were fabricated by host-guest interaction between cholesterol（Chol） and/3-CD. This study presented the influence of concentrations and molar ratio on mechani- cal strength, PLGA molecular weight on mechanical strength, pore size, swelling ratios and biodegradability. The results showed that storage modulus was highest at concentrations of 150 g/L and Chol/β-CD molar ratio of 1： 1. The average pore size of PLGA-based supermolecular hydrogels fabricated from concentrations of 150 g/L was between 10-30 μm. Swelling ratio was between 16%-32.5%. Degradation time could be controlled PLGA molecular weight from 40 d to 72 d. The excellent self-healing ability of hydrogels was performed. The cytotoxieity evaluation was observed that the adipose-derived stem cells treated with the polymers remained almost 100% viable, indicating good cytocompatibility of the hydrogels.

关 键 词：聚谷氨酸 Β-环糊精 胆固醇 超分子水凝胶 储存模量 生物相容性 

分 类 号：O636[理学—高分子化学]