Q-TOFLC/MS技术分析乙醇体积分数对人参单体皂苷提取率的影响  

Analysis of the Impact of Ethanol Volume Fraction on Extract Rate of Ginsenoside Monomer by Q-TOF LC/MS

作　　者：杨宗林[1] 李慧[1] 王冰[1] 刘淑莹[1,2] 

机构地区：[1]吉林省人参科学研究院/长春中医药大学,长春130117 [2]中国科学院长春应用化学研究所,长春130022

出　　处：《中国药房》2015年第34期4834-4836,共3页China Pharmacy

基　　金：吉林省科技发展计划项目(No.20130206059YY)

摘　　要：目的:研究乙醇体积分数对不同人参皂苷单体提取率的影响。方法:采用不同体积分数的乙醇为溶剂[0%(水)、20%、50%、70%、95%,V/V]对人参进行回流提取,经四极杆-飞行时间液相色谱/质谱(Q-TOF LC/MS)技术鉴定各峰的成分归属,并对其中的16种主要皂苷进行分析。结果:乙醇体积分数对人参药材中总皂苷的提取率存在较大影响,当乙醇体积分数为70%时总皂苷的提取率最高,此差别主要由三醇型皂苷引起,二醇型皂苷在不同体积分数乙醇溶液中的提取率基本相似。结论:乙醇体积分数不同时,得到提取物中的皂苷不仅在总量上存在差异,在单体比例上亦存在差异。OBJECTIVE：To study the impact of ethanol volume fraction on the extract rate of ginsenoside monomer. METHODS：Water and different volume fraction of ethanol solution（20%,50%,70%,95%,V/V）were used for ginseng extract. High performance liquid chromatography-quadrupole-time of flight-mass spectrometry（Q-TOF LC/MS） was applied to identify components attributable of each peak. Especially 16 kinds of main saponins were analyzed. RESULTS：The volume fraction of ethanol had a significant impact on extract rate of ginsenoside. When the volume fraction of ethanol was 70%,extract rate of ginsenoside was the highest;however,the differences were mainly caused by protopanaxatriol saponins. The yield of protopanaxadiol ginsenosides was similar in different volume fraction of ethanol solution. CONCLUSIONS：When the volume fraction of ethanol is different,result is not only different in the total saponin,but also different in the proportion of the monomers.

关 键 词：乙醇体积分数 人参 四极杆-飞行时间液相色谱/质谱 皂苷单体 提取率 

分 类 号：R283[医药卫生—中药学]