前列腺癌细胞转移相关基因的筛选  

Screening and identification of significant genes related to prostate tumor metastasis

作　　者：马建宏[1,2] 曹开源[1,3,4,5,6] 徐霖[3,5,6] 钟慧玲[1,3] 张素粉[1,3] 罗虹娇 庹玖玲 张甜[1,3] 刘建中[4] 

机构地区：[1]中山大学临床检验标准化研究中心,广东广州510080 [2]广东省人民医院,广东广州510080 [3]中山大学中山医学院微生物学教研室,广东广州510080 [4]中山大学中山医学院生物学教研室,广东广州510080 [5]中山大学热带病防治研究教育部重点实验室,广东广州510080 [6]广东省重大传染病预防和控制中心,广东广州510080

出　　处：《热带医学杂志》2015年第11期1455-1459,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金(81071760;30772503)

摘　　要：目的通过对si RNA-PSMA-LNCa P、PC-3和LNCa P前列腺癌细胞株进行肿瘤转移基因芯片分析,筛选出与前列腺癌细胞转移相关的基因,并对芯片结果进行验证。方法利用肿瘤转移基因芯片技术,对si RNA-PSMALNCa P细胞株、PC-3细胞株和LNCa P前列腺癌细胞株进行肿瘤转移相关基因表达差异分析,从中筛选出可能调控前列腺癌细胞转移的基因,并利用real-time PCR的方法在前列腺特异膜抗原(PSMA)阳性/阴性的前列腺癌细胞株和临床样品中对筛选得到的基因进行进一步验证。结果从肿瘤转移基因芯片结果筛选得到了3种在PSMA表达抑制或不表达情况下显著上调的基因:MMP3、CDH6和MTSS1;3种基因的表达在PSMA阳性细胞株和PSMA阴性细胞株中与芯片结果一致;利用临床组织对肿瘤转移基因芯片结果验证,结果显示3种前列腺癌细胞转移相关基因的表达水平与前列腺癌临床分级和PSMA的表达呈负相关。结论发现了3种可能参与调控前列腺癌细胞转移的基因:MMP3、CDH6和MTSS1。它们的表达水平与前列腺癌临床分级、PSMA的表达呈负相关,可能参与了由PSMA介导的前列腺癌细胞的转移。Objective To screen genes associated with prostate cancer metastasis by prostate tumor cell line microarray analysis. Methods The expression of cancer metastasis associated genes in si RNA-PSMA-LNCap cell lines, PC-3 cell line and LNCa P cell lines were analyzed by tumor metastasis gene microarray analysis.The results of tumor metastasis gene microarray analysis in prostate-specific membrane antigen（PSMA）-positive or negative prostate cancer cell lines and clinical samples were verified by real-time PCR. Results Gene microarrayresults reveal that the expression levels of three genes were significantly increased when PSMA was silence or no expression ：MMP3,CDH6 and MTSS1; same results was found in PSMA-positive or negative prostate cancer cell lines. The expression levels of these three genes in clinical samples were in correlated with tumor metastasis gene microarray analysis. Results showed a negative correlation between their expression levels and prostate cancer clinical grading. Conclusion The MMP3, CDH6 and MTSS1 genes may play important roles in prostate cancer metastasis. There is a strong negative correlation between the expression levels of these three genes and prostate cancer clinical grading and PSMA expression.

关 键 词：前列腺癌 前列腺特异膜抗原 肿瘤细胞转移 肿瘤转移基因芯片 

分 类 号：R737.25[医药卫生—肿瘤]