诱导多能干细胞体外分化为雪旺细胞的研究进展  

RESEARCH ADVANCE OF DIFFERENTIATION OF INDUCED PLURIPOTENT STEM CELLS INTO Schwann CELLS IN VITRO

作　　者：李润欣[1] 刘华蔚[1] 鄢荣曾[1] 胡敏[1] 

机构地区：[1]中国人民解放军总医院口腔颌面外科,北京100853

出　　处：《中国修复重建外科杂志》2015年第12期1560-1563,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家自然科学基金资助项目(81371116);北京市自然科学基金资助项目(13G30867)~~

摘　　要：目的对近年诱导多能干细胞(induced pluripotent stem cells,i PS)体外诱导分化为雪旺细胞的相关研究进展进行综述。方法查阅目前i PS体外诱导分化为雪旺细胞的相关文献,对i PS体外分化为雪旺细胞的诱导方案以及对分化形成的雪旺细胞进行鉴定和功能性检测方法进行总结。结果研究表明i PS可诱导分化为雪旺细胞,但目前需要先将i PS诱导分化为神经嵴细胞或神经嵴干细胞,再将其进一步分化为雪旺细胞。S100-β和胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)为公认的雪旺细胞标记物。当神经嵴细胞或神经嵴干细胞分化前标记为p75^+、HNK1^+或nestin^+,诱导后标记为S100-β^+、GFAP^+,表明诱导产生的是雪旺细胞。结论将i PS诱导分化为雪旺细胞的研究虽然已有进展,但目前成功的诱导方案甚少,仍需对其进行深入研究。Objective To review the research advance of differentiation of induced pluripotent stem cells （iPS） into Schwann cells in vitro in recent years. Methods Related literatures on differentiation of iPS into Schwann cells in vitro at present were consulted, the induction methods of iPS differentiating into Schwann ceils in vitro were summarized, and the differentiated cells were identified and detected. Results The research results indicate that iPS can differentiate into Schwann cells. So far, the iPS have to differentiate into neural crest ceils or neural crest stem cells firstly, and then differentiate into Schwann cells. S 100-β and glial fibrillary acidic protein （GFAP） are recognized as the marker of Schwann cells. The evidence of generating Schwann cells was that the neural crest cells or neural crest stem cells were labelled by p75＋, HNK1＋, or nestin＋ before differentiation, and by S100-β＋ and GFAP＋ after induction. Conclusion Despite the increasing reported studies of Schwann cells from iPS, there have been few successful induction methods, so this field of cytology needs further study.

关 键 词：诱导多能干细胞 神经嵴 雪旺细胞 分化 鉴定 

分 类 号：R741[医药卫生—神经病学与精神病学]