平喘宁对哮喘大鼠肺组织MEK1、MEK2、Ras mRNA表达的影响  

Effect of Pingchuanning on mRNA Expression of MEK1,MEK2,and Ras in Lung Tissues from Asthmatic Rats

作　　者：方向明[1] 朱佳琪[1] 邬锡琴[2] 

机构地区：[1]安徽中医药大学,安徽合肥230038 [2]合肥工业大学,安徽合肥230009

出　　处：《安徽中医药大学学报》2015年第6期57-61,共5页Journal of Anhui University of Chinese Medicine

基　　金：国家自然科学基金项目(81173187)

摘　　要：目的观察平喘宁对寒性哮喘大鼠气道形态学及肺组织中丝裂原活化蛋白激酶的激酶(mitogen-activated protein kinase kinase,MEK)1mRNA、MEK2mRNA及Ras mRNA表达水平的影响。方法将105只雄性SD大鼠随机分为正常组,模型组,桂龙咳喘宁组,地塞米松组,平喘宁高、中、低剂量组,每组15只。以卵蛋白致敏及寒冷刺激复制大鼠哮喘模型,模型复制21d后,分别给予地塞米松组,桂龙咳喘宁组,平喘宁高、中、低剂量组大鼠相应药物灌胃,给予正常组和模型组大鼠等容量蒸馏水灌胃。4周后取出肺组织,采用苏木精-伊红染色行病理形态学观察,采用逆转录-聚合酶链式反应半定量检测MEK1mRNA、MEK2mRNA及Ras mRNA表达水平。结果与正常组比较,模型组发生明显炎性细胞浸润,气道平滑肌增厚;MEK1mRNA、MEK2mRNA以及Ras mRNA表达水平显著増高(P<0.05)。与模型组比较,地塞米松组、桂龙咳喘宁组、平喘宁组大鼠肺组织病理改变减轻;各治疗组MEK1mRNA、MEK2mRNA以及Ras mRNA表达水平显著降低(P<0.05)。结论平喘宁可明显减轻哮喘大鼠的气道炎性反应,抑制哮喘大鼠肺组织中MEK1mRNA、MEK2mRNA以及Ras mRNA的表达,延缓气道重塑而治疗哮喘。Objective To investigate the effect of Pingchuanning on airway morphology and the mRNA expression of mitogen-activated protein kinase kinase 1 （MEK1）, mitogen-activated protein kinase kinase 2 （MEK2）, and Ras in the lung tissues of rats with cold-type asthma. Methods One hundred and five male Sprague-Dawley rats were randomized into normal group, model group, Guilongkechuanning group, dexamethasone group, and high-, medium-, and low-dose Pingchuanning groups, with 15 rats in each group. A rat model of asthma was established through ovalbumin sensitization and cold stimulation; 21 days later, the rats in the dexamethasone group, the Guilongkechuanning group, and the high-, medium-, and low-dose Pingchuanning groups were given respective drugs by gavage, and those in the normal group and the model group were given an equal volume of distilled water by gavage. The lung tissues were collected 4 weeks later, and hematoxylin and eosin staining was applied to observe the pathomorphological changes. Reverse transcription-polymerase chain reaction was applied for semiquantitative determination of mRNA expression levels of MEK1, MEK2, and Ras. Results Compared with the normal group, the model group had obvious inflammatory cell infiltration and airway smooth muscle thickening and significantly higher mRNA expression levels of MEK1, MEK2, and Ras （P〈0.05）. Compared with the model group, the dexamethasone group, the Guilongkechuanning group, and the Pingchuanning groups had fewer histopathological changes in lung tissues; each treatment group had significantly reduced mRNA expression levels of MEK1, MEK2, and Ras after treatment （P〈0.05）. Conclusion Pingchuanning can alleviate the airway inflammatory response, inhibit the mRNA expression of MEK1, MEK2, and Ras, delay airway remodeling, and thus reach the purpose of treating asthma.

关 键 词：哮喘 平喘宁 MEK1 MEK2 RAS 

分 类 号：R562.2[医药卫生—呼吸系统]