不同蛋白质和小麦淀粉水平对大黄鱼生长性能、糖酵解和糖异生关键酶活性的影响  

Effects of dietary protein and wheat starch levels on growth performance,hepatic glycolysis and gluconeogenic key enzymes activities in large yellow croaker( Larimichthys crocea Richardson)

作　　者：王猛强 黄文文[1] 周飘苹 金敏[1] 邱红[1] 周歧存[1] 

机构地区：[1]宁波大学海洋学院,浙江宁波315211

出　　处：《水产学报》2015年第11期1690-1701,共12页Journal of Fisheries of China

基　　金：国家自然科学基金(31272670);宁波市农业科技攻关重大项目(2012C10025)~~

摘　　要：本实验旨在研究不同蛋白质和小麦淀粉水平对大黄鱼生长性能、肝脏糖酵解和糖异生关键酶活性、血清指标、糖原含量及消化酶活性的影响。采用2×3双因素实验设计,共配制6组饲料,包含2个蛋白质水平(41%、46%)和3个小麦淀粉水平(10%、20%、30%),选取初重约为(14.84±0.16)g的大黄鱼900尾,随机分为6组(每组3个重复,每个重复50尾),进行8周的养殖实验。实验结果表明:饲料蛋白质和小麦淀粉水平的交互作用对大黄鱼增重率和特定生长率无显著影响(P>0.05),且在相同的饲料蛋白质水平下,饲料小麦淀粉水平对大黄鱼增重率和特定生长率无显著影响(P>0.05)。饲料蛋白质和小麦淀粉的交互作用对大黄鱼肝脏6-磷酸果糖激酶(phosphofructokinase,PFK)、丙酮酸激酶(pyruvate kinase,PK)、果糖-1,6-二磷酸酶(fructose-1,6-bisphosphatase,FBPase)活性有显著影响(P<0.05),但对葡萄糖激酶(glucokinase,GK)、葡萄糖-6-磷酸酶(glucose-6-phosphatase,G6Pase)、磷酸烯醇式丙酮酸羧激酶(phosphoenolpyruvate,PEPCK)活性无显著影响(P>0.05)。当饲料蛋白质水平为41%时,饲料小麦淀粉水平对葡萄糖激酶、6-磷酸果糖激酶和果糖-1,6-二磷酸酶活性有显著影响(P<0.05),且果糖-1,6-二磷酸酶活性随着饲料小麦淀粉水平的升高呈下降趋势,但对丙酮酸激酶、葡萄糖-6-磷酸酶、磷酸烯醇式丙酮酸羧激酶活性无显著影响(P>0.05);当饲料蛋白质水平为46%时,饲料小麦淀粉水平对葡萄糖激酶、6-磷酸果糖激酶、丙酮酸激酶、果糖-1,6-二磷酸酶活性有显著影响(P<0.05),且葡萄糖激酶、6-磷酸果糖激酶、丙酮酸激酶均随饲料小麦淀粉水平的升高呈上升趋势,但对葡萄糖-6-磷酸酶和磷酸烯醇式丙酮酸羧激酶活性无显著影响(P>0.05)。实验表明,大黄鱼在高蛋白质水平下能够有效调节糖酵解和糖异生关键酶活性,降低血糖含量及丙氨酸转氨酶、天冬氨酸转氨酶活性,并且�An 8-week feeding trial was conducted to evaluate the effects of different dietary protein and wheat starch levels on growth performance,hepatic metabolic enzymes activities,hematological characteristics,glycogen content and digestive enzyme activities in large yellowcroaker. Six experimental diets were formulated to contain two protein levels（ 41% and 46%） and three wheat starch levels（ 10%,20% and 30%）. Each diet was randomly assigned to 3 replicates of 50 juvenile large yellowcroaker[initial weight approximately（ 14. 84 ± 0. 16） g]. The results indicated that weight gain rate and specific growth rate were not significantly affected by the interaction of dietary protein and wheat starch levels（ P〈0. 05）. When the dietary protein level was the same,dietary wheat starch levels had no significant effect on WGR and SGR（ P〉0. 05）. However,the interaction of dietary protein and wheat starch levels had significant effects on phosphofructokinase,pyruvate kinase,fructose-1,6-bisphosphatase（ P〈0. 05）,but there were no significant influences on glucokinase,glucose-6-phosphatase,phosphoenolpyruvate in liver（ P〉0. 05）. When the dietary protein level was 41%,glucokinase,phosphofructokinase and fructose-1,6-bisphosphatase were significantly affected by dietary wheat starch levels（ P〈0. 05）,and fructose-1,6-bisphosphatase significantly decreased with increase of dietary wheat starch levels,but had no significant effects on pyruvate kinase,glucose-6-phosphatase,phosphoenolpyruvate in liver（ P〉0. 05）. When dietary protein level was 46%,glucokinase,phosphofructokinase,pyruvate kinase and fructose-1,6-bisphosphatase were significantly affected by wheat starch levels（ P〈0. 05）,and glucokinase,phosphofructokinase,pyruvate kinase increased with dietary wheat starch levels increasing from 20% to 30%. The results indicated that,when the dietary wheat starch level is high,large yellowcroaker has the ability to maintain blood glucose content by adjusting the hepatic glycogen,glycolys

关 键 词：大黄鱼 蛋白质水平 小麦淀粉水平 生长性能 糖酵解 糖异生 

分 类 号：S963.7[农业科学—水产养殖]