新疆Ⅱ、Ⅲ期宫颈癌患者外周血T细胞亚群、B细胞、NK细胞分析  

Analysis of T cell subsets,B cells and NK cells on peripheral blood in patients with Ⅱ andⅢstage cervical cancer in Xinjiang District

作　　者：姜鹏[1,2] 胡云辉[1,2] 古丽娜.库尔班 王若峥[1,2] 

机构地区：[1]新疆医科大学附属肿瘤医院放疗中心,新疆乌鲁木齐830011 [2]新疆医科大学附属肿瘤医院新疆肿瘤学重点实验室,新疆乌鲁木齐830011 [3]新疆医科大学附属肿瘤医院妇瘤放疗科

出　　处：《重庆医学》2015年第34期4796-4798,共3页Chongqing medicine

基　　金：科技部国际合作基金资助项目(2012DFA31560)

摘　　要：目的探讨T细胞亚群、B细胞、NK细胞的比例在新疆地区宫颈癌患者中的特点。方法随机抽取临床Ⅱ、Ⅲ期宫颈癌患者140例,健康人群159例作为对照,运用流式细胞术检测外周血中T细胞亚群、B细胞、NK细胞的比例。结果宫颈癌CD3^+T细胞、CD8^+T细胞、NK细胞的比例均低于健康人群;CD4^+T细胞、CD4^+/CD8^+T细胞比例高于健康人群,差异均有统计学意义(P<0.05)。两组间B细胞比较差异无统计学意义(P>0.05)。维吾尔族宫颈癌CD3^+T细胞、CD8^+T细胞、NK细胞比例显著低于健康人群,CD4^+T细胞、CD4^+/CD8^+T细胞比例显著高于健康人群;汉族宫颈癌仅CD3^+T细胞比例显著低于健康人群;所有细胞比例在维、汉之间均无显著差异。HPV16^+组与HPV16-组之间,CD4^+T细胞、NK细胞比例比较差异有统计学意义(P<0.05)。T细胞亚群、B细胞、NK细胞在不同年龄、不同临床分期间比较差异无统计学意义(P>0.05)。结论新疆维吾尔族Ⅱ、Ⅲ期宫颈癌患者外周血T细胞亚群、NK细胞与健康人群存在差异,且可能与HPV表达有关。Objective To investigate the ratio characteristic of T cell subsets,B cells and NK cells in patients with cervical cancer in Xinjiang Uygur Autonomous Region. Methods Randomly selected 140 patients with Ⅱ and Ⅲ cervical cancer as experi- mental group and 159 healthy people as control group. The proportion of T cell subsets,B cells and NK cells in peripheral blood were detected by means of flow cytometry. Results The CD3^＋ T cells,CD8^＋ T cells and NK cells of patients with U , lIl cervical cancert were lower than healthy controls,while the proportions of CD4^＋ T cells and CD4^＋/CDS＋ T cells were higher than healthy controls,the differences were statistically significant（P〈0.05）. There was no statistically significant difference between the two groups about B cells（P〉0.05）. The percentage of CD3^＋ T cells,CD8^＋ T cells and NK cells in Uighur patients with cervical cancer significantly lower than those of healthy people, while CD4^＋ T ceils ratio and CD4^＋/CD8^＋ T cell ratio were significantly higher than healthy people. The ratio of CD3^＋T cells in Han patients with cervical cancer was significantly lower than those of health people, but there were no difference between Han and Uygur. There were significant differences between HPV16 positive and HPV16 negative,as well as in CD4^＋T cells and NK cells（P〈0.05）. There were no statistical differences among T cell subsets,B cells and NK cells in different ages and stages. Conclusion There exists differences between patients with Ⅱ and Ⅲ stage cervical cancer in Xinjiang and healthy people about T cell subsets and NK cells of peripheral lymphocytes,which may be related to HPV expression.

关 键 词：宫颈肿瘤 免疫细胞 T淋巴细胞亚群 NK细胞 

分 类 号：R737.33[医药卫生—肿瘤]