低温等离子体联合辐射对三种癌细胞系放射增敏效应的研究  

Radiosensitization effect of low-temperature plasma on human malignant cells

作　　者：胡超[1] 钱丹琪[2] 秦颂兵[1] 叶超[3] 周菊英[1] 

机构地区：[1]苏州大学附属第一医院,215006 [2]江南大学附属医院 [3]苏州大学物理学院

出　　处：《中华放射医学与防护杂志》2015年第11期819-824,共6页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的探讨低温等离子体对人肝癌细胞系HepG2、非小细胞肺癌细胞系A549及人宫颈癌细胞系HeLa的放射增敏作用及其机制。方法应用克隆形成实验观察低温等离子体对3种细胞的放射增敏作用；流式细胞仪分析3种细胞周期分布、凋亡率及活性氧含量；Western blot法检测单纯照射（R组）、单纯等离子体作用（P组）及其联合辐射（P＋R组）对3种细胞中Bcl-2、Caspase-3蛋白表达的影响。结果低温等离子体对HepG2、A549及HeLa细胞均有放射增敏作用，放射增敏比（SER。。）分别为1．28、1．32、1．29。HepG2、A549及HeLa细胞P＋R组G2／M期比例、凋亡率及活性氧含量与R组比较均明显增高（tG2／M期=9．52、8．24、9．53，P〈0．05；t凋亡率=10．67、38．56、6．74，P〈0．05；t活性氧含量=9．41、15．42、13．53，P〈0．05）。HepG2和A549细胞P＋R组G2／M期比例、凋亡率及活性氧含量与P组比较均明显升高（tG2／M期=8．75、20．37，P〈0．05；t凋亡率=8．43、9．99，P〈0．05；t活性氧含量=4．82、5．27，P〈0．05）。3种癌细胞中P＋R组Bcl-2蛋白表达较R组降低，而Caspase-3蛋白表达升高。结论低温等离子体可提高HepG2、A549及HeLa细胞系的放射敏感性，其对HepG2及A549细胞系的放射增敏机制可能与抑制亚致死损伤修复，使细胞周期阻滞在G2／M期，以及提高细胞内ROS水平诱导细胞凋亡有关。Objective To evaluate the radiosensitization effect of low-temperature plasma on HepG2, A549, and HeLa cells. Methods Ceils were divided into three groups, radiation group（R） , plasma treatment group（P） , and plasma plus radiation group （P ＋ R）. After radiation, cell survival was detected by a cloning assay. Cell cycle distribution, apoptosis and ROS content were tested by flow cytometry. Western blot was used to measure the expressions of Caspase-3 and Bcl-2. Results Low- temperature plasma showed radiosensitization effects on three different human malignant cell lines with a sensitivity enhancement ratio（ SERo0 ） of 1.28,1.32 and 1.29. respectively. In these three different human malignant cell lines, compared with radiation alone group （R） , the G2/M arrest, apoptosis rate and ROS level in the group P ＋ R were enhanced （the prolongation of G2/M arrest： t = 9.52, 8.24, 9.53, P 〈 0.05; the apoptosis rate： t = 10.67, 38.56, 6.74, P 〈0.05; ROS content： t =9.41, 15.42, 13.53, P 〈 0.05）. In HepG2 cells and A549 cells, compared with group P, the prolongation of Ga/M arrest, the apoptosis rate and ROS content of group P ＋ R were enhanced （the prolongation of G2/M arrest ： t = 8.75, 20.37, P〈0.05; the apoptosis rate： t =8.43, 9.99, P 〈0.05; ROS content： t =4.82, 5.27, P 〈 0. 05）. The expression level of Bcl-2 protein was downregulated in group P ＋ R; by contrast, the expression level of Caspase-3 protein in group P ＋ R was upregulated. Conclusions Low-temperature plasma can increase the radiosensitization of HepG2, A549 and HeLa cells with the enhancement of G2/M phase arrest, apoptosis induction and ROS generation.

关 键 词：放射增敏 低温等离子体 HepG2细胞 HELA细胞 A549细胞 

分 类 号：R730.55[医药卫生—肿瘤]