基于荧光图像的快速检测藻类浓度方法的研究  

Study on Fast Detection Method of Algae Concentration based on Fluorescence Images

作　　者：徐元哲[1] 徐云升[2] 谢鑫刚[1] 吴路光 吕健威 

机构地区：[1]琼州学院电子通信学院,海南三亚572020 [2]琼州学院食品学院,海南三亚572020 [3]东北电力大学自动化工程学院,吉林吉林132013

出　　处：《东北电力大学学报》2015年第5期71-75,共5页Journal of Northeast Electric Power University

基　　金：海南省科技兴海专项资金项目(XH201316)

摘　　要：针对传统近海藻类监测的局限性,提出了一种新的藻类监测的方法。利用435 nm的激光器作为激发光源,得到波长为685 nm左右的藻类荧光图像,以发出的荧光强度图像的灰度值大小来表征水中藻类的浓度。通过实验确定了激发激光器的功率以及检测样品室的高度,结果表明,合理设计激发激光器的功率与检测样品室的高度,可以得到藻类浓度与荧光图像的灰度值之间的线性关系,为无人驾驶环境监测船提供了新的藻类检测方法。Aimed at the limitations of traditional offshore algae monitoring,this paper proposes a new method of algae monitoring. Using 435nm laser as the excitation light source,fluorescence images of algae at wavelength 685nm were obtained,the concentration of algae in the water was characterized by gray value of the fluores-cence intensity. The power of the excited laser and the height of the sample chamber were determined by exper-iments,the results show that it can get the relationship between algae concentration and the gray value of fluo-rescence image is linear through reasonable design of the power and height of the sample chamber. It provides a new detecting method for unmanned environment surveillance ship.

关 键 词：藻类浓度 叶绿素A浓度 荧光灰度 图像处理 

分 类 号：X834[环境科学与工程—环境工程] X832