重楼皂苷Ⅱ对人皮肤鳞状细胞癌细胞A431增殖的影响  

Effect of Polyphyllin Ⅱ on A431 Human Squamous-cell Carcinoma Cells

作　　者：姜福琼[1] 王晓琼[1] 刘馨[1] 张佩莲[1] 周晓鸿[1] 王文芳[1] 毕云凤[1] 

机构地区：[1]昆明医科大学第二附属医院,云南昆明650101

出　　处：《昆明医科大学学报》2015年第12期21-24,共4页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(2013C248)

摘　　要：目的研究重楼提取物单体重楼皂苷Ⅱ在体外对A431人皮肤鳞状细胞癌细胞增殖的影响.方法采用MTT法检测重楼皂苷Ⅱ对体外培养的A431人皮肤鳞状细胞癌细胞株增殖的影响;透射电镜观察细胞超微结构的变化;采用流式细胞术检测重楼皂苷Ⅱ干预后A431人皮肤鳞状细胞癌细胞增殖周期变化的情况.结果重楼皂苷Ⅱ对A431人皮肤鳞状细胞癌细胞的增殖有明显的抑制作用,重楼皂苷Ⅱ对A431细胞增殖的抑制作用呈现出浓度依赖性和时间依赖性(P<0.05).与此同时,重楼皂苷Ⅱ引起皮肤鳞状细胞癌细胞A431的超微结构发生变化,并将A431皮肤鳞状细胞癌细胞周期阻滞于S期(P<0.05).结论重楼皂苷Ⅱ对A431人皮肤鳞状细胞癌有潜在的抗癌作用,可能是通过对皮肤鳞状细胞癌细胞周期的阻滞及引起细胞凋亡而发挥作用.Objective To investigate the potential effects of polyphyllin Ⅱ（PP Ⅱ ） on A431 human squamous-cell carcinoma cells. Methods Human squamous cell carcinoma cell line A431 were cultured with or without PPⅡ. The cell proliferation was measured by MTT assay. The change of cell morphology was observed by transmission electron microscopy. The change of cell cycles was measured by flow cytometry. Results The proliferation of A431 human squamous-cell carcinoma cells was significantly inhibited by PP Ⅱ with dose- and time-dependent manners. The ultrastructure changes of A431 cells were observed. The cell cycles of A431 human squamous-cell carcinoma cells were arrested in S phase. Conclusion Polyphyllin Ⅱ have potentially anticancer effect on A431 human melanoma ceils in vitro. Inducing the cell cycle arresting and apoptosis of cells may be the potential mechanisms.

关 键 词：皮肤鳞状细胞癌 重楼皂苷Ⅱ 中药 

分 类 号：R739[医药卫生—肿瘤]