荧光定量聚合酶链式反应和基因芯片分型法行HPV分型检测的比较研究  

Comparative study on fluorescent quantitation polymerase chain reaction and gene-chips typing method in genotyping HPV

作　　者：龙秀荣 兰建云[2] 耿建祥[2] 阚延静[2] 范雪梅[2] 夏林[2] 王宏景[2] 梅静[2] 赵雪[2] 

机构地区：[1]南京市六合区人民医院病理科,江苏南京211500 [2]南京中医药大学第三附属医院病理科/江苏省HPV协作组,江苏南京210001

出　　处：《国际检验医学杂志》2015年第23期3385-3387,共3页International Journal of Laboratory Medicine

基　　金：南京市卫生局中医专项支助项目(2009-92)

摘　　要：目的比较荧光定量聚合酶链式反应(简称荧光定量法)与基因芯片分型法(简称基因芯片法)在检测人乳头瘤病毒(HPV)中的灵敏度,分析两者的差异及临床意义。方法以246例女性为研究对象,其中111例行液基细胞学诊断、135例行组织学诊断,采用荧光定量法和基因芯片法检测所有受试者15种高危HPV基因型,并进行比较分析。结果荧光定量法检测HPV的灵敏度为55.28%,基因芯片法灵敏度为55.69%,二者比较,差异无统计学意义(P>0.05);两种方法检测结果有较高的一致性(κ=0.745);HPV阳性率随宫颈病变程度增加而上升。结论荧光定量法和基因芯片法检测HPV具有较高的一致性,两者检测灵敏度都较高,宫颈病变的严重程度与HPV感染呈正相关。Objective To compare the sensitivity of fluorescent quantitation polymerase chain reaction（fluorescent quantitation method）and gene-chips typing method（gene-chips method）in the detection of human papillomavirus（HPV）,and to analyse differences and clinical significance.Methods A total of 246 women were selected as subjects,among them,111 cases of cervical exfoliated cells and 135 cases of cervical tissues were collected and detected.15 kinds of high-risk HPV genetypes were detected in all subjects by using fluorescent quantitation method and gene-chips method respectively,and the detection results were compared.Results The sensitivity of the fluorescent quantitation method in detecting HPV was 55.28% and that of the gene-chips method was 55.69%,there was no statistically significant difference in sensitivity between the two methods（P〈0.05）.The two methods had relative high conformance（κ=0.745）.The positive rate of HPV infection was increased with the progression of cervical disease.Conclusion The fluorescent quantitation method and the gene-chips method have a relative high conformance,and both with high sensitivity in detecting HPV.The severity degree of cervical cytological and histological changes may be positively correlated with HPV infection.

关 键 词：人乳头瘤病毒 荧光定量聚合酶链式反应 基因芯片分型法 

分 类 号：R737.33[医药卫生—肿瘤] R440[医药卫生—临床医学]