精神分裂症患者免疫球蛋白核心岩藻糖基化组分的富集纯化及免疫学性质分析  

A study on enrichment and purification of core fucose glycosylation and its immunity properties in patients with schizophrenia

作　　者：郭静[1] 邱锦云[1] 冯方波[1] 

机构地区：[1]解放军第二六一医院检验科,北京10094

出　　处：《国际检验医学杂志》2015年第23期3433-3434,共2页International Journal of Laboratory Medicine

摘　　要：目的建立富集纯化精神分裂症患者免疫球蛋白核心岩藻糖基化组分的技术平台,分析其免疫学性质。方法采用蛋白G交联琼脂糖凝胶分离纯化血清免疫球蛋白G（IgG）,小扁豆凝集素（LCA）亲和层析、富集纯化IgG中核心岩藻糖基化组分,免疫印迹分析LCA-IgG的IgG亚型。结果血清IgG纯化率为62%,LCA-IgG层析得率为4.59%。精神分裂症患者LCAIgG中可标记到IgG_1、IgG_2、IgG_3亚型,IgG_4未检出;而健康者LCA-IgG可标记到IgG1~IgG4全部4个亚型。结论成功建立了富集纯化精神分裂症患者IgG核心岩藻糖基化组分的技术平台,揭示了患者LCA-IgG亚型的分布特点及其与健康者的异同,为进一步研究患者的免疫病理机制提供了新的思路。Objective To establish a platform for enrichment and purification of core fucose glycosylation and analyse its immunity properties.Methods Serum immunoglobulin G（IgG）was seperated and purified by using protein G cross-linked sepharose,and core fucose glycosylation components of IgG were enriched and purified by using lens culinaris agglutinin（LCA）affinity chromatography,meanwhile,the isoforms of LCA-IgG were determined by using Western blotting.Results The purification rate of serum IgG was 62% and yield rate of LCA-IgG was 4.59%.Serum IgG1,IgG2 and IgG3 were identified except IgG4 in patients with schizophrenia,while serum IgG1,IgG2,IgG3 and IgG4 were all identified in healthy individuals.Conclusion A method for enrichment and purification of core fucose glycosylation in patients with schizophrenia have been successfully established and distribution characteristics of the LCA-IgG isoforms have been determined,which could provide a new insight into immune pathomechanism of schizophrenia.

关 键 词：精神分裂症 免疫球蛋白 核心岩藻糖基化 免疫球蛋白G亚型 

分 类 号：R749.3[医药卫生—神经病学与精神病学]