鞘内注射罗哌卡因对神经病理性痛大鼠脊髓HDAC1和HDAC2表达的影响  

Effect of intrathecal ropivacaine on spinal HDAC1 and HDAC2 expression in rats with neuropathic pain

作　　者：陈旦[1] 翁莹琪[1] 欧阳碧函[2] 郭绵星 郭曲练[1] 

机构地区：[1]中南大学湘雅医院麻醉科,长沙市410008 [2]中南大学湘雅医院健康管理中心,长沙市410008 [3]Department of Celluar and Molecular Biology, University of Carnifornia, Berkeley

出　　处：《中华麻醉学杂志》2015年第9期1093-1095,共3页Chinese Journal of Anesthesiology

基　　金：中南大学2012年度中央高校基本科研业务费青年教师助推专项资助课题

摘　　要：目的 评价鞘内注射酰胺类局麻药罗哌卡因对神经病理性痛大鼠脊髓组蛋白去乙酰化酶1（HDAC1）和HDAC2表达的影响.方法 成年雄性SD大鼠,体重220～ 250 g,取鞘内置管成功的大鼠30只,采用随机数字法分为3组（n=10）：假手术（S组）、神经病理性痛组（NP组）和神经病理性痛＋罗哌卡因组（R组）.采用坐骨神经慢性压迫性损伤（CCI）法制备大鼠神经病理性痛模型.CCI术后第7天起,R组鞘内注射0.25％罗哌卡因20 μl,S组和CCI组鞘内注射生理盐水20μl,1次/d,连续7d.分别于CCI术前1 d（T0）和CCI术后3、7、10、14、17和21 d（T1-6）时,测定机械缩足反应阈（MWT）和热缩足潜伏期（TWL）.于T4时痛阈测定结束后,每组取3只大鼠,采用Western blot法检测脊髓HDAC1和HDAC2的表达水平.结果 与S组比较,NP组和R组T2-6时MWT降低,TWL缩短,T4时脊髓HDACI和HDAC2的表达上调（P＜0.05）;与NP组比较,R组T3,4时MWT升高,TWL延长,T4时脊髓HDAC1和HDAC2的表达下调（P＜0.05）.结论 鞘内注射罗哌卡因减轻大鼠神经病理性痛的机制可能与抑制脊髓HDAC1和HDAC2表达上调有关.Objective To evaluate the effect of intrathecal ropivacaine on spinal histone deacetylase 1 （HDAC1） and HDAC2 expression in the rats with neuropathic pain （NP）.Methods Thirty adult male Sprague-Dawley rats, weighing 220-250 g, in which intrathecal catheters were successfully placed, were randomly divided into 3 groups （n=10 each） using a random number table： sham operation group （group S）, group NP, and NP ＋ ropivacaine group （group R）.NP was induced by chronic constriction injury （CCI） in anesthetized rats.Sciatic nerve was exposed and 4 loose ligatures were placed on the left sciatic nerve at 1 mm intervals with 4-0 chromic catgut.Starting from 7th day after CCI, 0.25% ropivacaine 20 μl was injected intrathecally in group R, while the equal volume of normal saline was given instead of ropivacaine in S and CCI groups once a day for 7 consecutive days.At 1 day before CCI （T0） ,and 3, 7, 10, 14, 17 and 21 days after CCI （T1-6） , the mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured.After the pain threshold was measured at T4,3 rats from each group were sacrificed, and their lumbar enlargements were harvested for determination of the expression of HDAC1 and HDAC2 by Western blot.Results Compared with group S, the MWT was significantly decreased, and the TWL was shortened at T2-6, and the expression of HDAC 1 and HDAC2 was up-regulated at T4 in NP and R groups （P〈0.05）.Compared with group NP, the MWT was significantly increased at T4, and the TWL was prolonged at T3.4, and the expression of HDAC1 and HDAC2 was downregulated at T4 in group R （P〈0.05）.Conclusion The mechanism by which intrathecal ropivacaine alleviates NP may be related to inhibited up-regulation of spinal HDACI and HDAC2 expression in rats.

关 键 词：组蛋白脱乙酰基酶类 脊髓 酰胺类 注射 脊髓 神经痛 

分 类 号：R741[医药卫生—神经病学与精神病学]