化学修饰的凋亡细胞诱导小鼠异体皮肤移植耐受的研究  

Study on chemically modified apoptotic donor cells induced immune tolerance in skin allografts of mice

作　　者：丁健科[1] 苏映军[1] 张栋梁[1] 陈晨[1] 陈建武[1] 余州[1] 宋雅娟[1] 庄然[2] 郭树忠[1] 

机构地区：[1]第四军医大学西京医院全军整形外科研究所,陕西西安710032 [2]第四军医大学基础部免疫教研室

出　　处：《中国美容整形外科杂志》2015年第12期761-764,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金资助项目（30830102）

摘　　要：目的研究ECDI处理的脾细胞对同种异体皮片存活的影响，并初步探讨其机制。方法建立小鼠尾部全厚皮片移植模型，随机法分5组。control组：免疫排斥组；rapa组：单独使用3mg／kg雷帕霉素组；ECDI．SPstwice组：移植前7d及移植后1d输注10^8 ECDI—SPs联合雷帕霉素组；SPs组：移植前7d及移植后1d输注未经处理脾细胞联合雷帕霉素组；ECDI—SPsx3组：移植前7d、移植后1d及7d3次输注ECDI—SPs联合雷帕霉素组。结果ECDI处理的脾细胞迅速发生了凋亡，ECDI—SPs两次输注明显延长了同种异体小鼠皮片存活时间（P〈0．05），ECDI—SPs3次输注皮片存活同2次输注差异无统计学意义（P〉0．05）。ECDI-SP输注后小鼠体内调节性T细胞比例增加（P〈0．05）。结论ECDI-SPs联合雷帕霉素能延长同种异体小鼠皮片存活时间，可能机制与调节性T细胞升高有关。Objective To investigate the effect of ECDI-SPs on the survival of a full-thickness skin allograft and its mechanism. Methods Full-thickness skin transplantation was established and mice were divided randomly into five groups; Control group： the skin allograft without treatment; Rapa group： skin allograft with 3 mg/kg rapamycin for 28 days ; ECDI-SPs twice group ： ECDI-treated splenocytes were injected i.v. on day 7 before （ - 7） and 1 day after （ ＋ 1 ） skin transplantation with 3 mg/kg rapamycin for 28 days ; SPs group ： Untreated splenocytes were injected the same way and time as ECDI-SPs twice group with 3 mg/kg rapamycin for 28 days; ECDI-SPs x3 group： Another time of ECDI-treated splenocytes were injected 7 day after skin transplantation on the basis of ECDI-SPs twice group. Results Infusions of donor splenocytes treated with ECDI prolonged skin allograft survival significantly （ P 〈 0.05 ）, and there were no significant difference between ECDI-SPs twice and x3 group （ P 〉 0.05）, the proportion of regulatory T cells increased in ECDI-SPs twice and x3 group （ P 〈 0.05）. Conclusion Pre-and post-transplant infusions of splenocytes treated with ECDI prolonged skin allograft survival probably by up-regulation of regulatory T cells in spleen.

关 键 词：乙基碳二亚胺 凋亡 皮片移植 复合组织移植 免疫耐受 雷帕霉素 

分 类 号：R-322[医药卫生]