纤维素选择性酸水解废液发酵制备乙醇工艺的研究  

Fermentation of Spent Liquor from Selective Acid Hydrolysis of Cellulose

作　　者：强丹丹 张美云[1] 李金宝[1] 张向荣[1] 

机构地区：[1]陕西科技大学轻工与能源学院陕西省造纸技术及特种纸品开发重点实验室,陕西西安710021

出　　处：《造纸科学与技术》2015年第5期77-81,93,共6页Paper Science & Technology

基　　金：教育部高校博士点专项基金(20126125130001);陕西省自然科学基金(2013JM2007);陕西科技大学博士启动基金(BJ13-02);陕西科技大学创新基金资助项目

摘　　要：酸水解木质纤维素制备微晶纤维素的过程中会产生一定量的废液,此废液中含有大量的还原糖。本课题研究了Fe3+助HCl选择性水解纤维素制备微晶纤维素过程中产生的废液,用于发酵制备生物乙醇,探索其发酵可行性。利用响应曲面分析法考察了酿酒酵母接种量、发酵温度、还原糖浓度、发酵时间四个工艺参数对响应值乙醇浓度的影响,优化发酵工艺参数,最终确定合理工艺条件。研究结果表明:当酿酒酵母接种量10.46%,发酵温度40℃,还原糖浓度60g/L,发酵时间42h时,乙醇浓度实际值可达15.6245g/L,乙醇得率为26.04%,此废液用于发酵制备乙醇的工艺可行。A plenty of reducing sugar existed in the spent liquor produced from acid hydrolysis treatment in preparation of microcrystalline cellulose. The spent liquor from acid hydrolysis selectivity of cellulose enhanced by metal ion after recycling for several times was investigated to explore the feasibility of fermentation. After investigating the four parameters,including saccharomyces cerevisiae inoculum,fermentation temperature,reducing sugar concentration,duration time,on the effect response value of ethanol concentration by response surface method,the result showed the theoretical concentration of ethanol could be up to 16. 0378g·L^-1 when the optimum fermentation conditions were the saccharomyces cerevisiae inoculum 10. 46%,fermentation temperature 40℃,reducing sugar concentration 60g·L^-1,duration time 42 h.

关 键 词：纤维素 酸水解废液 发酵 生物乙醇 响应面法 

分 类 号：TS79[轻工技术与工程—制浆造纸工程]