血吸虫病抗体检测试纸条对实验感染水牛的抗体检测  

Detection of Anti-schistosome Antibodies of Experimentally Infected Buffalo Using Rapid Diagnostic Strips

作　　者：阳爱国[1] 周煜 郭莉[1] 董国栋[3] 毛光琼[1] 邓永强[1] 田慧云 吴云飞 侯巍[1] 马坤[3] 陈冬[1] 文豪[1] 朱银宝 

机构地区：[1]四川省动物疫病预防控制中心,四川成都610041 [2]湖北省动物疫病预防控制中心,湖北武汉430000 [3]云南省动物疫病预防控制中心,云南昆明650051 [4]四川省广汉市动物疫病预防控制中心,四川广汉618003 [5]南京路宝生物科技有限公司,江苏南京210000

出　　处：《动物医学进展》2015年第12期113-116,共4页Progress In Veterinary Medicine

摘　　要：为明确牛血吸虫病抗体检测试纸条（以下简称试纸条）的检出时限,用试纸条对实验感染血吸虫的10头水牛的抗体消长情况进行了检测。结果显示,6头试验水牛（编号为1号、2号、4号、6号、7和9号）在接种后第4周检测到血吸虫抗体,4头试验水牛（编号为3号、5号、8号和10号）在接种第5周检测到血吸虫抗体。经过吡喹酮治疗后,3头试验水牛（1号、3号和8号）在第16周抗体转阴,4头试验水牛（2号、5号、6号和10号）在第20周抗体转阴,3头试验水牛（4号、7号和9号）在第24周抗体转阴。结果表明,该试纸条最早可检测到水牛血吸虫抗体的时间在感染后第4周,要保证全部检出的时间须在感染后第5周;经治疗后,试验水牛血液中抗体转阴的最早时间是治疗后16周,全部转阴的时间在治疗后24周。说明该试纸条用于水牛血吸虫病早期诊断时间应在感染后4周~5周,用于治疗药物的疗效评价应在患血吸虫病水牛治疗6个月后进行。In order to research the limit of the rapid diagnostic strip to detect buffalo antibodies against Schistosoma japonicum,the strip was used to detect the anti-schistosome antibodies of 10 experimental buffalo.The results showed that six experimental buffalo（the number 1,2,4,6,7and 9）had antischistosome antibodies after infection with schistosome cercaria 4 weeks,four experimental buffalo（the number 3,5,8and 10）had antibodies after infection 5weeks.The anti-schistosome antibodies of three experimental buffaloes（the number 1,3and 8）could not be detected after treatment with praziquantel for16 weeks,four experimental buffalo（the number 2,5,6and 10）could not be detected after treatment 20 weeks,and the others could not be detected after treatment 24 weeks.The results suggested that the antischistosome antibodies in buffalo could be detected after infection 4weeks;all buffalo could be detected in5 weeks.The anti-schistosome antibodies in buffalo sera could not be detected after treatment 16weeks;all anti-schistosome antibodies could not be detected after treatment 24 weeks.It is declared that the best time of rapid diagnostic strip for animal schistosomiasis（RDSAS）used to early diagnosis of schistosomiasis is after infection 4to 5weeks,the assessable therapeutic effect of medicine would be carried out after the livestock infection with schistosome 6months late.

关 键 词：抗体检测试纸条 水牛 血吸虫抗体 检测 

分 类 号：S852.7[农业科学—基础兽医学]