GRP78蛋白在鼻咽癌C666-1放射存活亚克隆中的表达  

Expression of GRP78 in the radiated survival subclones of nasopharyngeal carcinoma C666-1 cells

作　　者：张芳芳[1,2] 姜武忠[1] 李成敏[3] 冯雪萍[3] 

机构地区：[1]中南大学湘雅医院肿瘤科,长沙410008 [2]山西省晋城市人民医院肿瘤科 [3]中南大学湘雅医院医学实验研究中心

出　　处：《中国医师杂志》2015年第11期1620-1622,1627,共4页Journal of Chinese Physician

基　　金：国家自然科学基金资助项目（81372905）;湖南省自然科学基金资助项目（2015JJ4075）

摘　　要：目的 分析C666-1放射存活亚克隆的放射敏感性并分析其GRP78蛋白的表达水平.方法 首先使用大剂量8Gy的X射线照射C666-1,三个存活亚克隆被挑选,并被命名为C666-1-R1、C666-1-R2和C666-1-R3;然后通过MTT和台盼蓝染色法分析细胞系的放射敏感性,应用western blot分析三个存活亚克隆及母本C666-1的GRP78表达水平.结果 在6Gy放射后,三个亚克隆的细胞存活率均高于母本细胞株C666-1,尤其C666-1-R2显著增高（P＜0.05）;另一方面,各亚克隆的GRP78表达均高于母本C666-1（P＜0.05）.结论 放射存活亚克隆C666-1-R2具有放射抵抗性,GRP78在存活亚克隆中高表达;GRP78可能是鼻咽癌治疗的一个理想靶标.Objective To investigate radio-sensitivity and expression of GRP78 protein in the survival subclones of nasopharyngeal carcinoma （NPC） C666-1 cells.Methods NPC C666-1 cells were first irradiated with X-ray at a large dose of 8Gy.Three survival subclones were selected and named as C666-1-R1, C666-1-R2, and C666-1-R3.The radio-sensitivity was analyzed for the radiated survival subclones and their parent control C666-1 cells with Methyl Thiazolyl Tetrazolium assay（MTT） and Trypan blue dye methods.The expression of GRP78 was analyzed for three survival subclones and control C666-1 with Western blot.Results After 6 Gy irradiation, the cell survival rate of three subclones was higher than that of the control cells, especially a significant difference for C666-1-R2 cells （P 〈 0.05）, which suggested a radioresistance in C666-1-R2 cells.Moreover, GRP78 expression in each subclone was significantly higher than that of parent C666-1 cells （P 〈 0.05）.Conclusions The irradiated-survival subclone C666-1-R2 was radio-resistant.GRP78 was overexpressed in the irradiated-survival subclones.GRP78 might be an ideal target for treatment of a nasopharyngeal carcinoma.

关 键 词：膜蛋白质类/生物合成 鼻咽肿瘤/放射疗法/代谢 辐射耐受性 克隆 分子 

分 类 号：R739.63[医药卫生—肿瘤]