未成熟大鼠癫癎模型血清神经元特异性烯醇化酶、S-100B蛋白、胶质纤维酸性蛋白表达变化及意义  

Study on the changes and significance of serum NSE, S-100B and GFAP in immature rat model of epileptic seizure

作　　者：刘芹[1] 汤继宏[2] 张兵兵[2] 朱玉霞[2] 

机构地区：[1]江苏省盐城市妇幼保健院儿科,224001 [2]苏州大学附属儿童医院神经内科,215025

出　　处：《中国现代神经疾病杂志》2015年第11期885-889,共5页Chinese Journal of Contemporary Neurology and Neurosurgery

基　　金：江苏省自然科学基金资助项目(项目编号:BK2011309);江苏省苏州市科技计划项目(项目编号:SYS201349)~~

摘　　要：目的观察未成熟癫癎大鼠血清神经元特异性烯醇化酶(NSE)、S-100B蛋白(S-100B)、胶质纤维酸性蛋白(GFAP)表达变化,探讨其对未成熟脑癫癎发作的意义。方法采用三氟乙醚诱导建立未成熟大鼠癫癎模型,按照随机数字表法随机分为对照组和惊厥组(单次惊厥组和反复惊厥组),酶联免疫吸附试验分别检测大鼠发作第1,2,7和15天时血清NSE、S-100B、GFAP表达变化。结果与对照组相比,单次惊厥组大鼠于惊厥发作第1天血清NSE[(8.57±0.56)μg/L]和S-100B[(0.45±0.06)μg/L]表达水平即升高(均P=0.000),此后逐渐下降至正常水平(均P>0.05);反复惊厥组大鼠于惊厥发作第1天时血清NSE[(9.33±0.61)μg/L]和S-100B[(0.78±0.10)μg/L]表达水平即达峰值(均P=0.000),此后逐渐下降,至第7和15天时降至正常水平(均P>0.05),血清GFAP表达水平自反复惊厥发作第1天即升高[(0.44±0.05)μg/L,P=0.004],至第7天达峰值水平[(0.63±0.08)μg/L,P=0.000],此后逐渐下降但至第15天仍高于对照组[(0.40±0.05)μg/L,P=0.018]。结论 NSE和S-100B为单次惊厥发作性脑损伤的高敏感性生物学标志物,反复惊厥发作可使脑损伤程度加重,GFAP表达变化不具有脑损伤预测作用。Objective To observe the dynamic changes of neuron-specific enolase（NSE）,S-100 B protein（S-100B） and glial fibrillary acidic protein（GFAP） in the serum of immature epileptic rat model,so as to explore the significance of these biochemical indexes on the damage of immature brain after epileptic seizures.Methods The immature epileptic rat model was established by inducing flurothyl to 5-day-old specific pathogen free（SPF） Sprague-Dawley（SD） rats.The experimental animals were randomly divided into 2 groups：control group（N=8） and seizure group（N=64）.Furthermore,the latter was subdivided into single seizure group（N=32） and repeated seizures group（N=32）.Double antibody sandwich enzymelinked immunosorbent assay（ELISA） was used to detect the changes of serum NSE,S-100 B and GFAP in control group,single seizure group and repeated seizures group,on the 1st,2nd,7th and 15 th days of the seizure onset.Results Compared with control group,the concentrations of serum NSE and S-100 B in single seizure group increased significantly on the 1st day of onset（P =0.000,for all）,which respectively rose up to（8.57 ± 0.56） μg/L and（0.45 ± 0.06） μg/L,and then declined gradually to normal（P0.05,for all）,while no obvious changes of GFAP was found.Compared with control group,the concentrations of serum NSE and S-100 B in repeated seizures group reached the peak on the 1st day of onset[（9.33 ±0.61） μg/L and（0.78 ± 0.10） μg/L;P =0.000,for all],and then declined gradually to normal on the 7th and15 th days（P0.05,for all）.Compared with control group,the concentration of serum GFAP in repeated seizures group increased significantly on the 1st day of onset[（0.44±0.05） μg/L,P=0.004],reached the peak on the 7th day[（0.63±0.08） μg/L,P=0.000],then declined gradually,but was still significantly higher than that in control group on the 15 th day[（0.40±0.05）μg/L,P= 0.018].Conclusions NSE and S-100 B are highly sensitive biochemical markers of brain damage caus

关 键 词：癫癎 磷酸丙酮酸水合酶 S100蛋白质类 神经胶质原纤维酸性蛋白质 疾病模型 动物 

分 类 号：R742.1[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]