miRNA-218对裸鼠肾细胞癌调控作用的研究  

Effect of miRNA-218 on renal cell carcinoma in nude mice

作　　者：何龙[1] 刘龙[1] 杨宏伟[1] 张岩[2] 范连慧[1] 

机构地区：[1]沈阳军区总医院泌尿外科,110840 [2]沈阳军区总医院国家中医药重点实验室,110840

出　　处：《肿瘤研究与临床》2015年第11期728-731,共4页Cancer Research and Clinic

基　　金：吴阶平医学基金会临床科研专项基金（320.6750.13263）

摘　　要：目的观察miRNA-218对裸鼠肾细胞癌的作用。方法将pcDNA3．1-miR-218质粒及其对照空载体质粒分别转染至人肾癌细胞株A498、769．P，然后将转染后的细胞株按分组接种至裸鼠体内，观察裸鼠的机体及肿瘤的变化情况，并测定相应组织中miRNA-218的表达情况。结果A498＋pcDNA3．1．miR-218转染组荷瘤裸鼠在第25天开始体质量减轻程度均较对照组低，肿瘤体积在第10天后即较对照组小，差异均有统计学意义（均P〈0．05）；769-P＋pcDNA3．1-miR-218转染组荷瘤裸鼠在第19天开始体质量减轻程度均较对照组低，肿瘤体积在第10天后即较对照组小，差异均有统计学意义（均P〈0．05）。组织学检测两细胞miRNA-218转染组荷瘤裸鼠肿瘤组织miRNA-218表达量均较对照组增高，差异具有统计学意义（P〈0．05）。结论裸鼠体内实验证实，miRNA-218表达上调能够抑制肾肿瘤的生长。Objective To explore the effects of miRNA-218 on renal cell carcinoma of nude mice in vivo. Methods The pcDNA3.1-miR-218 and its control negative plasmids were stably transfected into renal cell carcinoma cell line A498 and 769-P. These cells were inoculated into nude mice in different groups to observe the changes of body and tumor and to detect the expression of miR-218 in the tissues of nude mice. Results In the A498 cells ＋ pcDNA3.1-miR-218 transfeeted group, the weight loss of tumor bearing nude mice after 25 days was lower than that in the control group, and the tumor volume was smaller than that in the control group after 10 days （P 〈 0.05）. In the 769-P cells ＋ pcDNA3.l-miR-218 transfected group, the weight loss of tumor bearing nude mice was lower than that in the control group after 19 days, and the tumor volume was smaller than that in control group after 10 days （P 〈 0.05）. The expression of miRNA-218 in bearing nude mice with A498 ceils or 769-P cells transfected by miRNA-218 was significantly higher than that in the control group, and the difference was statistically significant （P 〈 0.05）, Conclusion Up-regulation of miRNA-218 expression can inhibit the growth of renal cell carcinoma of nude mice in vivo.

关 键 词：肾肿瘤 miRNA-218 裸鼠 体内实验 抑癌基因 

分 类 号：R737.11[医药卫生—肿瘤]