MSCs对辐射诱导小鼠胸腺瘤抑制作用的实验研究  

Inhibitory effect of MSCs on radiation-induced thymoma in mice

作　　者：章文龙[1] 

机构地区：[1]惠州市第一人民医院心胸外科,广东惠州516003

出　　处：《海南医学院学报》2016年第1期5-8,共4页Journal of Hainan Medical University

基　　金：广东省惠州市科技计划项目(20130802)~~

摘　　要：目的:研究MSCs对辐射诱导小鼠胸腺瘤的抑制作用。方法:选择C57BL/6小鼠作为研究对象,模型组和MSCs组通过辐射的方式诱导小鼠产生胸腺瘤,MSCs组接受尾静脉注射间充质干细胞治疗。治疗后6个月,检测胸腺组织的宏观形态以及T淋巴细胞亚群含量、抑癌基因PTEN和增殖标志分子Ki-67的表达量。结果:(1)宏观形态:模型组小鼠的胸腺组织重量明显大于对照组,MSCs组小鼠的胸腺组织肿瘤低于对照组;(2)细胞和分子:与对照组比较,模型组小鼠胸腺组织中CD4^-CD8^-、CD4^+CD8^-、CD4^-CD8^+T细胞的含量以及PTEN的表达量明显减少,CD4^+CD8^+T细胞的含量以及Ki-67的表达量明显增加;与模型组比较,MSCs组小鼠胸腺组织中CD4-CD8-、CD4+CD8-、CD4-CD8+T细胞的含量以及PTEN的表达量明显增多,CD4^+CD8^+T细胞的含量以及Ki-67的表达量明显减少。结论:MSCs对辐射诱导小鼠胸腺瘤具有抑制作用,表现为促进CD4^+CD8^-和CD4^-CD8^+T细胞成熟、抑制细胞增殖。Objective： To study the inhibitory effect of MSCs on radiation-induced thymoma in mice. Methods： C57BL/ 6 mice were selected as the research objects. Rats in model group and MSCs group were induced thymoma by radiation, and MSCs group received intravenous injection of mesenchymal stem cell for therapy. Six months after treatment, macroscopic morphology of thymus tissue, contents of T lymphocyte subsets as well as expression of tumor suppressor gene PTEN and proliferation marker molecule Ki-67 were detected. Results： Thymus tissue weight of model group was significantly greater than that of control group and thymus tissue weight of MSCs group was lower than that of control group. Compared with con- trol group, contents of CD4 CD8 , CD4＋ CD8 and CD4-CD8＋ T cells as well as expression of PTEN in thymus tissue of model group significantly decreased, and CD4＋ CD8＋ T cell content as well as Ki-67 expression significantly increased compared with model group, contents of CD4-CDS-, CD4＋ CDS- and CD4 CD8＋ T cells as well as expression of PTEN in thymus tissue of MSCs group significantly increased, and CD4＋ CD8＋ T cell content as well as Ki-67 expression significantly decreased. Conclu- sion.. MSCs have inhibitory effect on radiation-induced thymoma in mice. They can promote CD4＋ CD8 and CD4-CD8＋ T cell maturation and inhibit cell proliferation.

关 键 词：胸腺瘤 间充质干细胞 T淋巴细胞 PTEN KI-67 小鼠 

分 类 号：R736.3[医药卫生—肿瘤]