高危型HPV阳性子宫颈癌组织和细胞株中TLR4/iNOS通路分子的表达及意义  

Role of TLR4/iNOS pathway molecules in high-risk HPV-positive cervical cancer tissue and cell lines

作　　者：王玎[1] 李志英[1] 卢娇[1] 

机构地区：[1]三峡大学仁和医院妇科,湖北宜昌443001

出　　处：《海南医学院学报》2016年第1期20-23,26,共5页Journal of Hainan Medical University

基　　金：湖北省教育厅科学技术研究计划重点项目(D20151202)~~

摘　　要：目的:研究高危型HPV阳性子宫颈癌组织和细胞株中TLR4/iNOS通路分子的表达及意义。方法:选择高危型HPV阳性的子宫颈癌患者35例和接受宫颈组织活检的35例健康者进行研究,分析宫颈组织中TLRs、NOS的mRNA含量;培养CaSki细胞株(HPV16阳性)、Hela细胞株(HPV18阳性)、C33a细胞株(HPV阴性),转染siRNA并测定TLR4、NF-kB、iNOS以及NO的含量。结果:高危型HPV阳性子宫颈癌组织中TLR4、iNOS的mRNA含量显著高于正常子宫颈活检组织(均P<0.05),TLR3、TLR7、TLR8、TLR9、eNOS、nNOS的mRNA含量与正常子宫颈活检组织比较无明显差异(P>0.05);CaSki细胞株和Hela细胞株中TLR4、NF-kB、iNOS的mRNA含量以及NO的含量均高于C33a细胞株(均P<0.05);转染TLR4的siRNA后,CaSki细胞株和Hela细胞株中NF-kB、iNOS的mRNA含量以及NO的含量均低于转染阴性对照siRNA(均P<0.05)。结论:高危型HPV阳性的子宫颈癌组织和细胞株中TLR4/iNOS通路分子表达增多,TLR4能够通过NF-kB来增加iNOS表达和NO生成,进而参与高危型HPV所致宫颈癌的病理过程。Objective： To study the expression of TLR4/iNOS pathway molecules in high-risk HPV-positive cervical cancer tissue and cell lines. Methods： A total of 35 cases with high-risk HPV-positive cervical cancer and 35 healthy subjects receiving cervical biopsy were enrolled for study, and mRNA contents of TLRs and NOS in cervical tissue were analyzed. CaS- ki cell lines （HPV16-positive）, Hela cell lines （HPV18-positive） and C33a cell lines （HPV-negative） were cultured, siRNA was transfected and contents of TLR4, NF-kB, iNOS and NO were detected. Results： mRNA contents of TLR4 and iNOS in high--risk HPV-positive cervical cancer tissue were significantly higher than those in normal cervical biopsy tissue, and com- parison of mRNA contents of TLR3, TLRT, TLR8, TLR9, eNOS and nNOS with normal cervical biopsy tissue showed no significant differences; mRNA contents of TLR4, NF kB and iNOS as well as NO levels in CaSki cell lines and Hela cell lines were higher than those in C33a cell lines. After transfection of TLR4 siRNA, mRNA contents of NF-kB and iNOS as well as NO levels in CaSki Cell lines and Hela cell lines were lower than those transfected with negative control siRNA. Conclusion： Expression of TLR4/iNOS pathway molecules in high--risk HPV-positive cervical cancer tissue and cell lines increases, and TLR4 can increase iNOS expression and NO generation through NF-kB, thus participate in pathological process of cervical cancer caused by high-risk HPV.

关 键 词：宫颈癌 人乳头瘤病毒 TOLL样受体 诱导型NOS 

分 类 号：R737.33[医药卫生—肿瘤]