二甲双胍对人滤泡状甲状腺癌细胞生长和能量代谢的影响  

The effect of metformin in growth and energy metabolism of follicular thyroid cancer cell

作　　者：张斯亮[1] 郭慧玲[1] 王红[1] 关海霞[1] 单忠艳[1] 滕卫平[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,内分泌研究所,辽宁省重点实验室,辽宁沈阳110001

出　　处：《现代肿瘤医学》2016年第2期190-194,共5页Journal of Modern Oncology

基　　金：中华医学会临床医学科研专项资金-施贵宝内分泌糖尿病研究项目(编号:13040480433)

摘　　要：目的:观察不同浓度二甲双胍对人滤泡状甲状腺癌(follicular thyroid cancer,FTC)细胞增殖、凋亡及能量代谢的影响。方法:体外培养人FTC细胞(FTC-133),分为对照组和不同浓度二甲双胍处理组(1、3和10mmol/L),分别培养24h、48h和72h。四甲基偶氮唑蓝(MTT)法检测细胞增殖,Annexin V-FITC/PI流式细胞术检测细胞凋亡,Seahorse能量代谢分析仪检测细胞糖酵解及线粒体呼吸的变化。结果:与对照组相比,二甲双胍抑制FTC-133增殖,随浓度及处理时间的增加,抑制效应加大,呈剂量-时间依赖性(P<0.05)。二甲双胍诱导FTC-133凋亡,随浓度及处理时间的增加,其中晚期凋亡率明显增加,呈剂量-时间依赖性(P<0.05)。二甲双胍可影响FTC-133糖代谢,与对照组相比,随二甲双胍浓度增加,糖酵解和线粒体呼吸可明显抑制,呈剂量-时间依赖性(P<0.05)。结论:二甲双胍抑制FTC-133增殖并诱导凋亡,其机制可能是影响癌细胞能量代谢进而抑制其能量供应。Objective： To investigate the effects of different concentration metformin on proliferation,apoptosis and energy metabolism of follicular thyroid cancer（ FTC）. Methods： After treating with metformin（ 0,1,3,10 mmol / L）,the viability of FTC cell line（ FTC- 133） was analyzed by 3-（ 4,5- dimethyl- thiazol- 2- yl）- 2,5- diphenyltetrazoliumbromide（ MTT）. Cell cycle analysis was performed by Annexin V- FITC / PI flow cytometry,the energy metabolism of FTC- 133 was tested by Seahorse. Results： Compared with control group,MTT assays showed that the proliferation of FTC- 133 was inhibited in a dose- time dependent manner after treating with metformin（ P〈0. 05）.Moreover,metformin induced the late apoptosis of FTC- 133（ P〈0. 05）. Metformin changed the pattern of cell energy metabolism,manifested with a significant decrease in glycolysis and mitochondrial respiration of FTC- 133 in a dose- time dependent manner（ P〈0. 05）. Conclusion： Metformin provided an anti- tumor effect in FTC- 133 which may be mediated by decreasing the energy supply of cancer cells.

关 键 词：二甲双胍 人滤泡状甲状腺癌 能量代谢 

分 类 号：R730.53[医药卫生—肿瘤] R736.1[医药卫生—临床医学]