黄芪多糖对胰岛素抵抗H9c2心肌细胞葡萄糖摄取的影响  

Effect of Astragalus Polysaccharide on Glucose Uptake of Insulin-resistant H9c2 Cardiomyocytes

作　　者：刘坚[1,2] 鲍芳[3] 柯玉叶[3] 杨政炜 张亚辉[1,2] 吴胜英[1] 

机构地区：[1]湖北医药学院基础医学院,湖北十堰442000 [2]湖北医药学院心血管生物研究室,湖北十堰442000 [3]湖北医药学院护理学院,湖北十堰442000 [4]湖北医药学院生物医学工程学院,湖北十堰442000

出　　处：《湖北医药学院学报》2015年第5期447-451,共5页Journal of Hubei University of Medicine

基　　金：湖北省自然科学基金项目(2015CFC809);湖北省教育厅科学研究计划项目(Q20142101);湖北医药学院研究生启动基金资助计划项目(2013QDJZR08);湖北医药学院学科建设基础研究探索项目(2014XKJSTS02)

摘　　要：目的:研究黄芪多糖(Astragalus polysaccharide,APS)对胰岛素抵抗H9c2(IR-H9c2)心肌细胞葡萄糖摄取的影响,并探讨其信号机制。方法:采用高浓度胰岛素处理复制IR-H9c2心肌细胞模型,给予一定剂量的APS处理,MTT法筛选合适的实验浓度,2-脱氧-[3H]-D-葡萄糖法检测细胞的葡萄糖摄取,Western blotting法检测腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)的磷酸化水平。结果:APS可以明显促进IR-H9c2心肌细胞对葡萄糖的摄取,并能显著提高其AMPK的活性,且AMPK抑制剂Compound C明显降低了APS刺激IR-H9c2心肌细胞葡萄糖摄取的作用。结论:APS可以促进IR-H9c2心肌细胞的葡萄糖摄取,这一作用与AMPK的活化有关。Objective To explore the effect of astragalus polysaccharide （APS） on glucose uptake of insulin-resistant H9c2 cardiomyocytes （IR-Hgc2） and its possible signal mechanism in vitro. Methods IR-Hgc2 models were induced by high concentration insulin. The models were cultured in a media containing APS. The activity of IR-H9c2 was detected by MTT. The glucose uptake was measured by the 2-deoxy-[3H]-D-glucose method. The phosphorylation of AMP-activated protein kinase （AMPK） was examined by Western blotting. Results APS could significantly increase the glucose uptake and AMPK activity of IR-Hgc2. The APS-stimulated glucose uptake was significantly attenuated by pretreatment with Compound C, a selective AMPK inhibitor. Conclusion APS could stimulate the glucose uptake in IR-H9c2 ,which may correlate with the activation of AMPK.

关 键 词：黄芪多糖 胰岛素抵抗 葡萄糖摄取 H9C2心肌细胞 

分 类 号：R285.5[医药卫生—中药学]