年龄因素对急性酒精性肝损伤和肝细胞凋亡的影响  

Impact of age on acute alcohol induced liver injury and hepatocyte apoptosis

作　　者：邱媛媛[1] 王伟恒[2] 胡江峰[3] 沈思[1] 朱樑[1] 

机构地区：[1]中国人民解放军第二军医大学长征医院消化内科,上海市200003 [2]中国人民解放军第二军医大学长征医院骨科,上海市200003 [3]上海同济大学附属同济医院消化内科,上海市200065

出　　处：《世界华人消化杂志》2015年第32期5123-5132,共10页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.10972235~~

摘　　要：目的:研究年龄因素对急性酒精性肝损伤和肝细胞凋亡的影响.方法:月龄为1、6、18 mo的SD大鼠每组各15只,随机分成实验组(n=10)和对照组(n=5),实验组每只大鼠以15 g/kg 400 mL/L乙醇一次性灌胃,对照组以等比例生理盐水一次性灌胃.灌胃48 h后取大鼠血液和肝脏组织分别行肝功能和ELISA检测肝脏炎症因子表达及TUNEL检测肝细胞凋亡情况.并进一步研究酒精刺激后肝细胞中Bcl-2、Bax、Caspase3等在凋亡通路中的表达情况,研究年龄因素对急性酒精性肝损伤和肝细胞凋亡的影响及可能机制.结果:对照组内,不同年龄组间肝功能、炎症指标无明显差异,月龄为1mo和6mo组肝细胞凋亡率无明显差异,18mo组高于其他两组.实验组内不同年龄组的肝功能、炎症因子表达和肝细胞凋亡率均显著高于对照组.且不同年龄组间不尽相同:实验组6mo的炎症因子表达高于其他组.而实验组内月龄为1mo的大鼠细胞凋亡率最高,6 mo次之,18mo较低.进一步研究显示Bcl-2、Bax、Caspase3在不同年龄实验组中表达不同,但同凋亡结果相一致.结论:生理状态下,肝功能、炎症因子与年龄大多无显著相关性,肝细胞凋亡率在老年时有所增加.急性过量饮酒可引起肝脏损伤和凋亡,且损伤程度和凋亡与年龄呈相关性:月龄为6 mo组肝脏损伤较重,而1mo组凋亡率更高.这可能与不同年龄大鼠在酒精刺激下肝脏炎症细胞的激活量及肝细胞凋亡通路的激活不同有关.AIM：To evaluate the effect of age on acute alcoholic liver injury and hepatocyte apoptosis.METHODS：Three age groups of SD rats（1-month,6-month and 18-month-old rats;n=15 each） were randomly divided into an experimental group（n=10） and a control group（n=5）.The experimental group was intragastrically given alcohol（40%V/V,15g/kg）,and the control group was given equal volume of normal saline.All the rats were sacrificed at 48 h to collect liver tissue and blood samples.Blood samples were tested for liver function,and the hepatic tissues were used for light microscopy,TUNEL,and ELISA for inflammatory cytokine measurement.RTPCR was used to detect the mRNA expression of Bcl-2,Bax,and Caspase3.RESULTS：In the control group,different age groups showed no significant difference in inflammation or liver function,although the liver cell apoptosis rate increased in the18-month age group.The experiment group showed significant differences in inflammation and liver function compared to the control group.In the experiment groups,the above indexes also showed significant differences among different age groups,with the 1-month group having the highest levels,which is consistent with the degree of damage.CONCLUSION：Under physiological conditions,liver function and inflammatory factors have no correlation with age,while apoptosis rate increases with age.Acute excessive alcohol consumption can cause liver damage,and the extent of damage is correlated with age,which may be related with different extents of inflammation and apoptosis in different age groups.

关 键 词：急性酒精性肝病 年龄 炎症因子 凋亡 肝功能 

分 类 号：R575[医药卫生—消化系统]