神经酰胺在非酒精性脂肪肝大鼠肝细胞脂质沉积中的作用  

Role of ceramide in hepatic lipid accumulation in rats with non-alcoholic fatty liver disease

作　　者：胡杨[1] 刘朝霞[1] 傅念[1] 吴清[1] 阳学风[1] 刘浩雷 

机构地区：[1]南华大学附属南华医院消化内科,湖南省衡阳市421002 [2]湖南中医药高等专科学校第一附属医院感染科,湖南省株洲市412000

出　　处：《世界华人消化杂志》2015年第32期5196-5200,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81373465~~

摘　　要：目的:探讨非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)大鼠肝内神经酰胺水平与肝细胞脂质沉积之间的关系.方法:将(?)SD大鼠随机分为正常对照组、NAFLD模型组和多球壳菌素组,NAFLD模型组采用高脂肪饲料喂养,多球壳菌素组除了高脂肪饲料喂养外还给予多球壳菌素喂养,正常对照组大鼠则喂养普通饲料.喂养12 wk后分别采用酶检测法、HE染色和高效液相色谱串联质谱法(high performance liquid chromatography tandem mass spectrometry,HPLC/MS)检测各组大鼠的肝组织脂质含量、脂质变性程度以及神经酰胺水平.结果:NAFLD模型组大鼠肝组织中的甘油三.酯和游离脂肪酸含量明显高于正常对照组和多球壳菌素组(P<0.05),而且模型组大鼠肝组织标本的脂肪变性程度均明显高于正常对照组和多球壳菌素组(P<0.05);与肝细胞内脂质沉积增加相应的是NAFLD模型组大鼠的肝内神经酰胺(主要是C24:0和C16:0神经酰胺)水平也明显高于正常对照组和多球壳菌素组(P<0.05).结论:神经酰胺参与了NAFLD大鼠肝细胞内的脂质沉积过程,抑制神经酰胺的合成则有助于减轻肝细胞内的脂质沉积程度.AIM：To evaluate the role of ceramide in hepatic lipid accumulation in rats with non-alcoholic fatty liver disease（NAFLD）.METHODS：Male SD animals were randomly divided into three groups：a control group,an NAFLD model group and a myriocin group.The NAFLD model group was fed a highfat diet,the myriocin group was fed a highfat diet and treated with myriocin,and the control group was fed a standard rodent diet only.After 12 wk,the liver tissues of rats in the three groups were measured for lipids levels,the extent of hepatic steatosis and ceramide levels by enzyme assay,HE staining and high performance liquid chromatography tandem mass spectrometry（HPLC/MS）,respectively.RESULTS：Compared with the control and myriocin groups,the levels of hepatic lipids,such as triglyceride and free fat acids,in the NAFLD model group were significantly increased（P〈0.05）,and the extent of hepatic steatosis in the NAFLD model group was significantly higher as well（P〈0.05）.Corresponding to lipid accumulation,the levels of ceramides in rat liver,mainly C24：0and C16：0 ceramides,were significantly higher in the NAFLD model group than in the control group and myriocin group（P〈0.05）.CONCLUSION：Ceramide contributes to hepatic lipid accumulation in rats with NAFLD,and inhibition of ceramide synthesis can reduce the extent of hepatic steatosis.

关 键 词：非酒精性脂肪肝 神经酰胺 脂质沉积 多球壳菌素 

分 类 号：R575.5[医药卫生—消化系统]