骨膜蛋白在子宫内膜癌的肿瘤干细胞中裸鼠成瘤性的实验研究  

Experimental study of periostin in the tumorigenesis of endometrial cancer stem cell for nude mice

作　　者：柯毓敏 周云清[1] 吴珠娜[1] 

机构地区：[1]福建医科大学附属第二医院妇科,福建泉州362200

出　　处：《中国临床药理学杂志》2015年第23期2329-2332,共4页The Chinese Journal of Clinical Pharmacology

基　　金：福建省卫生厅青年基金资助项目(2013-2-47)

摘　　要：目的探讨骨膜蛋白在子宫内膜癌的肿瘤干细胞中裸鼠成瘤特性。方法用免疫磁珠技术分离出骨膜蛋白,分别在30只裸鼠的左、右腋背部,皮下注射骨膜蛋白阳性细胞、骨膜蛋白阴性细胞,用流式细胞仪检测后进行计数,观察其中骨膜蛋白阳性细胞的比例。结果在35.7%(5/14)裸鼠胞浆内有骨膜蛋白表达,在正常子宫内膜组织中未见阳性表达;注射骨膜蛋白阳性细胞裸鼠的肿块重量、体积及瘤体骨膜蛋白阳性表达显著高于注射骨膜蛋白阴性细胞的裸鼠(P<0.05)。结论骨膜蛋白阳性的子宫内膜癌细胞在裸鼠体内具有成瘤性,而骨膜蛋白阴性细胞不具有成瘤性。Objective To investigate the test of periostin in the tumorigenesis of endometrial cancer stem cell for nude mice. Methods Through immunomagnetic beads technique,periostin was extracted. Thirty nude mice adopted the subcutaneous injection of periostin＋cells and periostin-cells in the left and right armpit. Flow cytometry was used to make a count of periostin cells. The ratio of periostin＋cell was observed.Results The 35. 7%（ 5 /14） of nude mice had the periostin expression in the cytoplasm while periostin was not positively expressed in the normal endometrial tissue. The tumor weight,volume and positive expression of periostin for nude mice with injection of positive periostin cells was significantly higher than that of injection of negative periostin cells（ P〈0. 05）. Conclusion For nude mice,periostin＋is featured as tumorigenesis while periostin-will not generate the tumor.

关 键 词：子宫内膜癌 成瘤性 肿瘤干细胞 骨膜蛋白 

分 类 号：R737.33[医药卫生—肿瘤]