桑树病程相关蛋白基因MuPR1-2的克隆及功能分析  

Cloning and Functional Analysis of Mulberry Pathogenesis Related Protein Gene MuPR1-2

作　　者：张华梁[1] 王红[2] 刘琪[2] 赵亚楠[2] 赵怀宁[1] 莫瑶瑶[1] 盖英萍[2] 冀宪领[1,2] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]作物生物学国家重点实验室,山东泰安271018

出　　处：《蚕业科学》2015年第6期979-988,共10页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(No.30972366;31070573;311004-78);山东省自然科学基金项目(No.2015ZRB019NE;201-5ZRB019UE);山东省现代农业产业技术体系项目(No.鲁农科技字〔2014〕24号)

摘　　要：病程相关蛋白(pathogenesis-related proteins,PRs)是植物体内受病原物或其他环境因子胁迫而诱导表达的一类蛋白质,在植物抗病反应过程中起着非常重要的作用。在桑树叶片中扩增得到MuPR1-2基因(Gen Bank登录号:KC453994),该基因编码区全长609 bp,编码蛋白质含有202个氨基酸,具有一个典型的信号肽和PR蛋白结构域以及PR1蛋白所共有的α-β-α夹心结构。将Mu PR1-2与绿色荧光蛋白基因gfp融合转入拟南芥,通过对转基因拟南芥根尖的显微结构观察,证明MuPR1-2蛋白定位于细胞壁或分泌到细胞间隙。转入MuPR1-2基因的拟南芥对丁香假单胞菌番茄致病变种(Pst DC3000)和灰霉菌具有较强的抵抗能力,表明Mu PR1-2在植物防御功能中具有重要作用。Pathogenesis-related proteins （PRs） are a group of proteins in plants induced by pathogens and different en- vironmental stresses. They play an important role in plant defense against pathogens. A pathogenesis-related protein gene was cloned from mulberry leaf and named MuPR1-2 （GenBank accession number KC453994）. The full length of its coding region is 609 bp and encodes a protein of 202 amino acids. MuPR1-2 protein possesses one typical signal pep- tide, conserved domains of PR1 proteins and consensus α-β-α sandwich structure. After being fused with gfp gene, Mu- PR1-2 gene was transformed into Arabidopsis. Microstructure observation of transgenic Arabidopsis thaliana plant root tips showed that MuPR1-2 protein was distributed in cell wall or secreted into intercellular space. Transgenic Arabidopsis thaliana plants transformed with MuPR1-2 gene showed strong resistance to Pseudomonas syringae pv. tomato DC3000 （ Pst DC3000） and Botrytis cinerea, indicating that MuPR1-2 gene plays an important role in plant defense.

关 键 词：桑树 病程相关蛋白 MuPR1-2基因 基因克隆 转基因拟南芥 抗性 

分 类 号：S888.7[农业科学—特种经济动物饲养]