一株桑树内生真菌的鉴定及菌丝多糖对α-葡萄糖苷酶活性的抑制作用  

Identification of a Mulberry Endophytic Fungus and Inhibitory Effect of Its Mycelium Polysaccharide on α-glucosidase

作　　者：郑丽屏 马燕军[2] 张雨青[3] 

机构地区：[1]苏州大学金螳螂建筑与城市环境学院,江苏苏州215123 [2]苏州大学药学院,江苏苏州215123 [3]苏州大学基础医学与生物科学学院,苏州市蚕丝生物技术重点实验室,江苏苏州215123

出　　处：《蚕业科学》2015年第6期1062-1066,共5页ACTA SERICOLOGICA SINICA

基　　金：现代农业产业技术体系建设专项(No.CARS-22)

摘　　要：采用平板分离法从桑树枝条中分离获得一株产α-葡萄糖苷酶抑制剂的内生真菌SZ-2,对该菌株进行鉴定和α-葡萄糖苷酶抑制活性检测。根据SZ-2菌株的形态特征、培养特征和5.8S r DNA/ITS序列分析,将其鉴定为链格孢菌(Alternaria sp.)。采用水提醇沉法提取发酵培养SZ-2菌株的菌丝多糖,测定菌丝多糖对α-葡萄糖苷酶具有较明显的体外抑制活性,IC_(50)值为6.98 mg/m L,且抑制类型属于典型的竞争性抑制作用。初步认为有望利用该菌株开发出新型的α-葡萄糖苷酶抑制剂。Using plate isolation method, one mulberry endophytic fungal strain SZ-2 producing α-glucosidase inhibitor was previously obtained from mulberry shoots. In this study, SZ-2 strain was identified, and its inhibitory effect on α-glucosidase was determined. According to morphological features, cultural characteristics, and 5.8S rDNA/ITS sequence analysis, SZ-2 strain was identified to be Alternaria sp. The mycelium polysaccharide of SZ-2 strain was extracted by using water-extracting and alcohol-precipitating method. The results showed that polysaccharide extract from the fermentation broth of SZ-2 strain had obvious in vitro inhibitory activity on α-glucosidase （the half inhibitory concentration IC50 value was 6.98 mg/mL） in a typical competitive pattern. It is considered that SZ-2 strain could be used to develop new type α-Cllucosidase inhibitor.

关 键 词：桑树 内生真菌 链格孢属 多糖 Α-葡萄糖苷酶 竞争性抑制 

分 类 号：Q93[生物学—微生物学]