白细胞介素-11受体介导的放射性探针对前列腺癌的抑制作用  

Inhibition potential of a interleukin - 11 receptor - mediated radiolabeled molecule probe on pros- tare cancinoma cells

作　　者：孙晋[1] 陈道桢[1] 刘璐[2] 邵国强[3] 胡瑶[1] 李天女[1] 谢彦[2] 王强[2] 刘标[1] 

机构地区：[1]南京医科大学第一附属医院核医学科,210029 [2]东南大学附属中大医院 [3]南京医科大学

出　　处：《中华实验外科杂志》2015年第12期2946-2949,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（81202032、81372480、81301247）;高等学校博士学科点专项科研基金课题新教师类（20123234120016）

摘　　要：目的探讨白细胞介素-11受体（IL-11R）介导的放射性探针对前列腺癌细胞抑制作用。方法构建IL-11R仅亚基靶向的放射性标记探针；免疫荧光评估靶向载体修饰肽对前列腺癌细胞结合能力；细胞计数试剂盒（CCK-8）法、Transwell及划痕法检测放射性标记探针对前列腺癌细胞增殖及迁移抑制作用。结果成功构建131I-Tyr—CGRRAGGSC，放化纯达99％以上；修饰肽与前列腺癌细胞显著结合。1．85、3．70、7．40、1d．80MBq/ml剂量组对PC-3M、DU-145抑制率分别为（9．83±2．55）％、（22．20±2．31）％、（58．87±4．98）％、（65．24±4．99）％和（6．23±3．27）％、（19．48±3．89）％、（32．27±2．87）％、（58．13±4．45）％；Transwell实验示1．85、5．55MBq／ml剂量组迁移率分别为（75．22±6．27）％、（25．58±6．01）％，差异均有统计学意义（P〈0．05）。划痕实验亦证实迁移抑制。结论经IL-11Rα介导的131I—Tyr—CGRRAGGSC对前列腺癌细胞增殖及迁移的抑制作用明确。Corresponding author ： Liu Lu , Email ： luliuzhou@ sina. corn [ Abstract ] Objective To discuss the inhibitory effects of a interleukin - 11 receptor （ IL - 11R） - mediated radiolabeled probe on prostate cancer. Methods The radiolabeled probe was prepared. Immunoflu- orescence staining was performed to observe the binding status of modified CGRRAGGS. The effects of ra- diolabeled probe were explored on cell proliferation and migration by cell counting kit -8 （CCK -8）, Tr- answell and wound - healing assay. Results 1311 - Tyr - CGRRAGGSC was radiolabeled and radiochemical purity after purification was over 99%. The prostate cancer cells had high binding abilities to the modified peptides. Inhibition ratio of 1.85, 3, 70, 7. 40 and 14. 80 MBq/ml ∽3J I - Tyr - CGRRAGGSC was （9. 83 ± 2. 55）%, （22. 20 ±2. 31）%, （38.87 _±4.98）% and （65.24 ±4. 99） % respectively to PC - 3M cells, and （6.23 ±3.27）%, （19.48±3.89）%, （32.27 ±2.87）% and （58. 13 ±4.45）% respectively to DU - 145 cells. The migration ratio of 1.85 MBq/ml and 5.55 MBq/ml groups was （75.22 ±6. 27）% and （25.58±6. 01 ）%, respectively, confirmed by wound- healing assay. All the difference was statistically significant （P 〈 0. 05 ）. Conclusion Mediated by IL - 1 l receptor, the inhibitory effects of 1311 - Tyr - CGRRAGGSC have been proven on prostate cancer cell proliferation and migration.

关 键 词：前列腺癌 增殖 迁移 放射性标记 肿瘤治疗 

分 类 号：R737.25[医药卫生—肿瘤]