印度块菌(Tuber indicum)菌根促生细菌的研究  

Study on mycorrhization helper bacteria(MHB) of Tuber indicum

作　　者：王冉[1] 刘培贵[1] 万山平[1] 于富强[1] 

机构地区：[1]中国科学院昆明植物研究所资源植物与生物技术实验室,云南昆明650201

出　　处：《微生物学通报》2015年第12期2366-2376,共11页Microbiology China

基　　金：国家自然科学基金项目(No.31270075;31370070)

摘　　要：【目的】筛选对印度块菌菌根量和菌根苗长势有促进作用的菌根促生细菌（Mycorrhizationhelper bacteria, MHB）。【方法】选择华山松为宿主植物，自块菌菌根根际土壤中分离得到的11种细菌为供试菌株，将印度块菌菌剂与不同浓度的细菌混合于特定基质中后接种于华山松上，并通过对印度块菌与华山松形成的菌根数、华山松的株高和地径三方面的统计与分析，确认MHB。【结果】Pseudomonas sp. JCM 5481 （P143）, Streptomyees sp. EN31 （S191）, Variovoraxparadoxus （V633）在浓度为2．4×10^9CFU／mL时对印度块菌菌根数、株高和地径均有极显著促进作用（P〈0．01）；Pseudomonaschlororaphis（P11）、Pseudomonascorrugate（P127）在浓度为0．8×10^9CFU／mL时对印度块菌菌根数、株高和地径均有显著促进作用（p〈0．05）。4种假单胞菌浓度梯度的设置显示了不同菌株适宜的浓度不同。【结论】实验获得5种MHB，并表明细菌浓度是获得MHB的一个重要影响因素。[Objective] To screening mycorrhization helper bacteria （MHB） which were facilitation to the quantity of mycorrhiza and the growing of mycorrhizal seedlings. [Methods] Pinus armandii was an experimental tree species, and eleven bacteria were isolated from rhizosphere soil of Tuber indicum as experimental bacteria. T. indicum inocula and different concentration bacteria were inoculated on P. armandii seedlings. The MHB were ascertained by statistics and analysis of the quantity of mycorrhiza, plant height and ground diameter of the P. armandii mycorrhizal seedlings synthesized by T. indicum. [Results] Pseudomonas sp. JCM 5481 （P143）, Streptomyces sp. EN31 （S191） and Variovorax paradoxus （V633） of 2.4× 10^9 CFU/mL had highly significant role in promoting （P〈0.01） the quantity of mycorrhiza, plant height and ground diameter, as well as Pseudomonas chlororaphis （P11） and P. corrugate （P127） of 0.8×10^9 CFU/mL had significant role in promoting （P〈0.05） the quantity of mycorrhiza, plant height and ground diameter. Different concentration setting of four Pseudomonasshowed each strain has its suitable concentration respectively. [Conclusion] Five MHB were obtained in the experiment, and indicated that the concentration of bacteria was a key factor to get MHB.

关 键 词：印度块菌 菌根合成 菌根促生细菌 

分 类 号：Q93[生物学—微生物学]