复合酶法辅助提取刺五加叶中金丝桃苷工艺研究  

Research on Composite Enzyme Assisted Hyperoside Extraction of Acanthopanax senticosus and Its Process

作　　者：陈秋连[1] 郑曼玲[1] 蔡恩博[1] 赵岩[1] 赵天琦[1] 屠书梅 杨利民[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《世界科学技术-中医药现代化》2015年第9期1866-1871,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：吉林农业大学国家级大学生创新创业训练计划项目(2012023):复合酶联用大孔吸附树脂技术提取分离刺五加叶中金丝桃苷;负责人:陈秋连

摘　　要：目的:文章通过复合酶法辅助提取刺五加叶中的金丝桃苷,并利用正交试验优化其提取工艺。方法:利用高效液相色谱法,以金丝桃苷为检测指标,对加酶量、温度、时间和p H值等因素,采用正交试验及方差分析法,以确定金丝桃苷复合酶法最佳提取工艺。结果:单因素试验的结果表明,各种酶对金丝桃苷提取率均有一定程度的提高,各因素的影响水平从高到低分别为p H、中性蛋白酶、温度、时间、果胶酶、木聚糖酶和纤维素酶。通过正交试验分析可得:在复合酶为2%果胶酶、2%木聚糖酶、0.5%中性蛋白酶和0.5%纤维素酶,提取温度为30℃,提取为时间为10 min,p H=4.5时,提取效果最佳,提取率为1.84%,与传统工艺相比,金丝桃苷产率提高了107%。结论:使用复合酶法辅助提取金丝桃苷可较大的提高产率,具有一定经济价值。This study was aimed to optimize the extraction process of hyperoside from leaves of Acanthopanax senticosus by compounding-enzyme method and orthogonal experiment. The hyperoside compound was regarded as standard and determined by HPLC. Based on the experiments of 4 factors including the enzyme amount, temperature, extraction time and PH values, the extraction process of hyperoside was determined by the orthogonal experiments and variance analysis. The results of single-factor experiments showed that different enzymes showed different effects on the enhance yield of hyperoside. The effects of different factors showed that the order of PH, neutral protease, temperature, time, pectinase, xylanase and cellulose was from strong to weak. Through orthogonal analysis, the optimum conditions were 2% pectinase, 2% xylanase, 0.5% neutral protease, and 0.5% cellulose, under the temperature of 30°C, extraction time of 10 min, and p H = 4.5. Under these conditions, the extraction rate was 1.84%. The yield was increased 107% compared with traditional process. It was concluded that the use of compounding enzyme can increase the yield of hyperoside, which possessed a lot of economic benefits.

关 键 词：刺五加 金丝桃苷 正交试验 复合酶法 提取工艺 

分 类 号：R284.2[医药卫生—中药学]